Abstract
In vitro mass multiplication of Vanilla planifolia was investigated using node as explant. Multiple shoots were developed in MS medium supplemented with 2.0 mg l -1 6-benzylaminopurine and 1.0 mg l -1 -naphthalene acetic acid. Multiple shoots were maintained for 6-7 weeks with regular subculturing at the end of 3 rd week onto fresh medium. The maximum number of shoots at the rate of 12.8 per node segment was achieved over a period of four weeks. The elongated shoots were separated from the shoot clusters and were transferred onto half strength MS medium supplemented with indole-3-acetic acid (1.0 mg l -1 ) over a period of 28 days for induction of roots. The development of roots was observed on 7 th day of incubation. The in vitro raised plantlets were transferred to poly-cups, covered with polyethylene sheets and maintained under shade net for 25 days for hardening. Finally these plants were transferred to field and recorded that 85 % of tissue cultured plants were survived. From the present study, a simple and efficient micropropagation protocol was developed for Vanilla planifolia using single node segments as explants.
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Claims 5
Claim · model-written summary
Node explants developed multiple shoots on supplemented MS medium.
- Taxon
- Vanilla planifolia
Vanilla planifoliat0 - Treatment
- MS medium supplemented with 2.0 mg l -1 6-benzylaminopurine and 1.0 mg l -1 -naphthalene acetic acide0
- Outcome
- Multiple shootse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Multiple shoots were developed in MS medium supplemented with 2.0 mg l -1 6-benzylaminopurine and 1.0 mg l -1 -naphthalene acetic acid.”
t0“Vanilla planifolia”
Claim · model-written summary
The maximum shoot production was 12.8 per node segment in four weeks.
- Taxon
- Vanilla planifoliaVanilla planifoliat0
- Outcome
- number of shootse0
- Direction
- maximume0
- Value
- 12.8 per node segmente0
- Qualifier
- over a period of four weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum number of shoots at the rate of 12.8 per node segment was achieved over a period of four weeks.”
t0“Vanilla planifolia”
Claim · model-written summary
Roots appeared on the seventh day after incubation.
- Taxon
- Vanilla planifoliaVanilla planifoliat0
- Outcome
- development of rootse0
- Value
- 7 th day of incubatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The development of roots was observed on 7 th day of incubation.”
t0“Vanilla planifolia”
Claim · model-written summary
Eighty-five percent of tissue-cultured plants survived field transfer.
- Taxon
- Vanilla planifoliaVanilla planifoliat0
- Outcome
- tissue cultured plants were survivede0
- Value
- 85 %e0
- Setting
- fielde0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Finally these plants were transferred to field and recorded that 85 % of tissue cultured plants were survived.”
t0“Vanilla planifolia”
Claim · model-written summary
The study developed a simple and efficient node-based micropropagation protocol for Vanilla planifolia.
- Taxon
- Vanilla planifoliaVanilla planifoliae0
- Material
- single node segmentse0
- Outcome
- simple and efficient micropropagation protocole0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From the present study, a simple and efficient micropropagation protocol was developed for Vanilla planifolia using single node segments as explants.”