Abstract
Small RNAs (sRNAs), an important type of pathogenicity factor, contribute to impairing host immune responses. However, little is known about sRNAs in Puccinia striiformis f. sp. tritici (Pst), one of the most destructive pathogens of wheat (Triticum aestivum L.). Here, we report a novel microRNA-like RNA (milRNA) from Pst termed microRNA-like RNA 1 (Pst-milR1), which suppresses wheat defenses during wheat-Pst interactions. We identified Pst-milR1 as a novel milRNA in Pst. Biological prediction and co-transformation showed that Pst-milR1 takes part in cross-kingdom RNA interference (RNAi) events by binding the wheat pathogenesis-related 2 (PR2) gene. Silencing of the Pst-milR1 precursor resulted in increased wheat resistance to the virulent Pst isolate CYR31. PR2 knock-down plants increased the susceptibility of wheat to the avirulent Pst isolate CYR23. This suggests that Pst-milR1 represses the plant immune response by suppressing the expression of PR2. Taking our findings together, we postulate that Pst-milR1 is an important pathogenicity factor in Pst, which acts as an effector to suppress host immunity. Our results provide significant new insights into the pathogenicity of the stripe rust pathogen.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Pst-milR1 was identified as a novel pathogen milRNA.
- Taxon
- wheat
Triticum aestivumt0 - Interactor
- Pste0pathogen
- Gene
- Pst-milR1e0
- Outcome
- novel milRNA in Pste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified Pst-milR1 as a novel milRNA in Pst”
t0“impairs wheat resistance”
Claim · model-written summary
Pst-milR1 bound wheat PR2 in cross-kingdom RNA interference.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Pste1pathogen
- Gene
- wheat pathogenesis-related 2 (PR2) genee0
- Outcome
- cross-kingdom RNA interference (RNAi) eventse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pst-milR1 takes part in cross-kingdom RNA interference (RNAi) events by binding the wheat pathogenesis-related 2 (PR2) gene”
e1“Pst-milR1 as a novel milRNA in Pst”
Claim · model-written summary
Silencing the Pst-milR1 precursor increased wheat resistance to CYR31.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Silencing of the Pst-milR1 precursore0
- Interactor
- Pst isolate CYR31e0pathogen
- Outcome
- wheat resistancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of the Pst-milR1 precursor resulted in increased wheat resistance to the virulent Pst isolate CYR31”
Claim · model-written summary
PR2 knockdown increased wheat susceptibility to CYR23.
- Taxon
- wheatTriticum aestivume0
- Treatment
- PR2 knock-downe0
- Interactor
- Pst isolate CYR23e0pathogen
- Outcome
- susceptibility of wheate0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PR2 knock-down plants increased the susceptibility of wheat to the avirulent Pst isolate CYR23”
Claim · model-written summary
The authors infer that Pst-milR1 suppresses PR2 expression to repress wheat immunity.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Pste1pathogen
- Gene
- PR2e0
- Outcome
- plant immune responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pst-milR1 represses the plant immune response by suppressing the expression of PR2”
t0“impairs wheat resistance”
e1“Pst-milR1 as a novel milRNA in Pst”
The same questions in other papers
loss of function · disease · Triticum aestivum (wheat)Positive and negative results
80 papers in the same direction as this paper · 96 in the opposite direction
- negative
- negative
- positive
173 papers more in this group
See all 177 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.