Abstract
A tissue culture laboratory exercise illustrating regeneration of whole plants from leaf segments of Chrysanthemum by organogenesis will be described. Using simple, common media, shoots can be generated in five weeks and rooted after an additional three weeks. Acclimatization of plants can be accomplished in a simple mistbed in the greenhouse. The exercise is adaptable to depict genotype differences among cultivars, optimization of shoot induction, effects of growth regulators, and experimental design. Callus is typically not formed during shoot formation; however, co-cultivation of leaf segments with a virulent strain of Agrobacterium tumefaciens produces callus autotrophic for growth regulators. Co-cultivation with a strain of disarmed A . tumefaciens harboring a NPTII construct affects regeneration of plants resistant to kanamycin.
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Claims 6
Claim · model-written summary
Chrysanthemum leaf segments generated shoots in five weeks.
- Taxon
- CHRYSANTHEMUM
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Chrysanthemumt0 - Treatment
- simple, common mediae0
- Outcome
- shoots can be generatede0
- Value
- five weekse0
Not reportedOrgan / stage
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e0“Using simple, common media, shoots can be generated in five weeks and rooted after an additional three weeks.”
t0“CHRYSANTHEMUM CULTIVARS”
Claim · model-written summary
Shoots rooted after another three weeks.
- Taxon
- CHRYSANTHEMUMChrysanthemumt0
- Outcome
- rootede0
- Value
- an additional three weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoots can be generated in five weeks and rooted after an additional three weeks”
t0“CHRYSANTHEMUM CULTIVARS”
Claim · model-written summary
Plants were acclimatized in a greenhouse mistbed.
- Taxon
- CHRYSANTHEMUMChrysanthemumt0
- Outcome
- Acclimatization of plantse0
- Setting
- a simple mistbed in the greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acclimatization of plants can be accomplished in a simple mistbed in the greenhouse.”
t0“CHRYSANTHEMUM CULTIVARS”
Claim · model-written summary
Callus was typically absent during shoot formation.
- Taxon
- CHRYSANTHEMUMChrysanthemumt0
- Organ / stage
- shoote0
- Outcome
- Callus is typically not formede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus is typically not formed during shoot formation”
t0“CHRYSANTHEMUM CULTIVARS”
Claim · model-written summary
Virulent Agrobacterium tumefaciens co-cultivation produced growth-regulator-autotrophic callus.
- Taxon
- CHRYSANTHEMUMChrysanthemumt0
- Treatment
- co-cultivation of leaf segments with a virulent strain of Agrobacterium tumefaciense0
- Interactor
- Agrobacterium tumefaciens
Agrobacterium tumefaciense0pathogen - Outcome
- callus autotrophic for growth regulatorse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“co-cultivation of leaf segments with a virulent strain of Agrobacterium tumefaciens produces callus autotrophic for growth regulators”
t0“CHRYSANTHEMUM CULTIVARS”
Claim · model-written summary
Disarmed Agrobacterium tumefaciens carrying NPTII produced kanamycin-resistant regenerated plants.
- Taxon
- CHRYSANTHEMUMChrysanthemumt0
- Treatment
- Co-cultivation with a strain of disarmed A . tumefaciens harboring a NPTII constructe0
- Interactor
- A . tumefaciensAgrobacterium tumefaciense0other
- Gene
- NPTIIe0
- Outcome
- regeneration of plants resistant to kanamycine0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Co-cultivation with a strain of disarmed A . tumefaciens harboring a NPTII construct affects regeneration of plants resistant to kanamycin.”
t0“CHRYSANTHEMUM CULTIVARS”