Abstract
Wheat (Triticum aestivum L.) incurs significant yield losses from powdery mildew, a major fungal disease caused by Blumeria graminis f. sp. tritici (Bgt). enhanced disease resistance1 (EDR1) plays a negative role in the defense response against powdery mildew in Arabidopsis thaliana; however, the edr1 mutant does not show constitutively activated defense responses. This makes EDR1 an ideal target for approaches using new genome-editing tools to improve resistance to powdery mildew. We cloned TaEDR1 from hexaploid wheat and found high similarity among the three homoeologs of EDR1. Knock-down of TaEDR1 by virus-induced gene silencing or RNA interference enhanced resistance to powdery mildew, indicating that TaEDR1 negatively regulates powdery mildew resistance in wheat. We used CRISPR/Cas9 technology to generate Taedr1 wheat plants by simultaneous modification of the three homoeologs of wheat EDR1. No off-target mutations were detected in the Taedr1 mutant plants. The Taedr1 plants were resistant to powdery mildew and did not show mildew-induced cell death. Our study represents the successful generation of a potentially valuable trait using genome-editing technology in wheat and provides germplasm for disease resistance breeding.
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Claims 6
Claim · model-written summary
The three wheat EDR1 homoeologs were highly similar.
- Taxon
- wheat
Triticum aestivumt0 - Gene
- EDR1e0
- Outcome
- high similarity among the three homoeologs of EDR1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“found high similarity among the three homoeologs of EDR1”
t0“wheat”
Claim · model-written summary
Silencing TaEDR1 increased wheat resistance to powdery mildew.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Knock-down of TaEDR1 by virus-induced gene silencing or RNA interferencee0
- Gene
- TaEDR1e0
- Outcome
- resistance to powdery mildewe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knock-down of TaEDR1 by virus-induced gene silencing or RNA interference enhanced resistance to powdery mildew”
t0“wheat”
Claim · model-written summary
TaEDR1 negatively regulates wheat resistance to powdery mildew.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaEDR1e0
- Outcome
- powdery mildew resistancee0
- Direction
- negatively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaEDR1 negatively regulates powdery mildew resistance in wheat”
Claim · model-written summary
The edited Taedr1 wheat plants had no detected off-target mutations.
- Taxon
- wheatTriticum aestivumt0
- Material
- Taedr1 mutant plantse0
- Outcome
- No off-target mutations were detectede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No off-target mutations were detected in the Taedr1 mutant plants”
t0“wheat”
Claim · model-written summary
The Taedr1 wheat plants resisted powdery mildew.
- Taxon
- wheatTriticum aestivumt0
- Material
- Taedr1 plantse0
- Outcome
- resistant to powdery mildewe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Taedr1 plants were resistant to powdery mildew”
t0“wheat”
Claim · model-written summary
The Taedr1 wheat plants showed no mildew-induced cell death.
- Taxon
- wheatTriticum aestivumt0
- Material
- Taedr1 plantse0
- Outcome
- did not show mildew-induced cell deathe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Taedr1 plants were resistant to powdery mildew and did not show mildew-induced cell death”
t0“wheat”
The same questions in other papers
loss of function · disease · Triticum aestivum (wheat)Positive and negative results
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