Abstract
Haloragis eyreana is an endangered plant endemic to South Australia. In anticipation of the need to translocate plants into areas from which it has been extirpated, it was necessary to develop techniques for rapid vegetative propagation. Field-derived material was successfully decontaminated using HgCl, and NaOCt, and cultured on modified half-strength MS medium supplemented with 0.05 mg/l BA. Shoot proliferation was approximately l0-fold over 4 weeks, and 93oh of cultures initiated roots on the same medium over this time. Rooted plantlets were transferred to soil, and after acclimatization, over 987o survived. After 2 months plants were in flower, and appeared phenotypically similar to parent plants.
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Claims 6
Claim · model-written summary
Field-derived material was successfully decontaminated and cultured on supplemented medium.
Not reportedTaxon
Verbatim
e0“Field-derived material was successfully decontaminated using HgCl, and NaOCt, and cultured on modified half-strength MS medium supplemented with 0.05 mg/l BA”
Claim · model-written summary
Shoots proliferated approximately tenfold over four weeks.
Not reportedTaxon
Verbatim
e0“Shoot proliferation was approximately l0-fold over 4 weeks”
Claim · model-written summary
Ninety-three percent of cultures initiated roots on the medium over four weeks.
Not reportedTaxon
Verbatim
e0“93oh of cultures initiated roots on the same medium over this time”
Claim · model-written summary
More than 98% of acclimatized plantlets survived after transfer to soil.
Not reportedTaxon
Verbatim
e0“Rooted plantlets were transferred to soil, and after acclimatization, over 987o survived”
Claim · model-written summary
The propagated plants flowered after two months.
Not reportedTaxon
Verbatim
e0“After 2 months plants were in flower”
Claim · model-written summary
Propagated plants appeared phenotypically similar to parent plants.
Not reportedTaxon
Verbatim
e0“appeared phenotypically similar to parent plants”