Abstract
Accelerated breeding of plant species has the potential to help challenge environmental and biochemical cues to support global crop security. We demonstrate the over-expression of Arabidopsis FLOWERING LOCUS T in Agrobacterium-mediated transformed cassava (Manihot esculenta Crantz; cultivar 60444) to trigger early flowering in glasshouse-grown plants. An event seldom seen in a glasshouse environment, precocious flowering and mature inflorescence were obtained within 4–5 months from planting of stem cuttings. Manual pollination using pistillate and staminate flowers from clonal propagants gave rise to viable seeds that germinated into morphologically typical progeny. This strategy comes at a time when accelerated crop breeding is of increasing importance to complement progressive genome editing techniques.
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Claims 4
Claim · model-written summary
Arabidopsis FLOWERING LOCUS T overexpression triggered early cassava flowering.
- Taxon
- cassava
Manihot esculentae0 - Material
- cultivar 60444e0
- Treatment
- over-expression of Arabidopsis FLOWERING LOCUS Te0
- Gene
- Arabidopsis FLOWERING LOCUS Te0
- Outcome
- early floweringe0
- Direction
- earlye0
- Setting
- glasshouse-growne0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“over-expression of Arabidopsis FLOWERING LOCUS T in Agrobacterium-mediated transformed cassava (Manihot esculenta Crantz; cultivar 60444) to trigger early flowering in glasshouse-grown plants”
Claim · model-written summary
Transformed cassava produced precocious flowering and mature inflorescences within 4–5 months of stem-cutting planting.
- Taxon
- CassavaManihot esculentat0
- Organ / stage
- inflorescencee0
- Outcome
- precocious flowering and mature inflorescencee0
- Value
- 4–5 monthse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“precocious flowering and mature inflorescence were obtained within 4–5 months from planting of stem cuttings”
t0“Cassava (Manihot esculenta Crantz)”
Claim · model-written summary
Manual pollination of cassava clonal flowers produced viable seeds.
- Taxon
- CassavaManihot esculentat0
- Organ / stage
- pistillate and staminate flowerse0
- Treatment
- Manual pollinatione0
- Outcome
- viable seedse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Manual pollination using pistillate and staminate flowers from clonal propagants gave rise to viable seeds”
t0“Cassava (Manihot esculenta Crantz)”
Claim · model-written summary
The resulting cassava seeds germinated into morphologically typical progeny.
- Taxon
- CassavaManihot esculentat0
- Organ / stage
- seedse0
- Outcome
- germinated into morphologically typical progenye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“viable seeds that germinated into morphologically typical progeny”
t0“Cassava (Manihot esculenta Crantz)”