Abstract
Transcription factors play vital roles in plant growth and in plant responses to abiotic stresses. The RAV transcription factors contain a B3 DNA binding domain and/or an APETALA2 (AP2) DNA binding domain. Although genome-wide analyses of RAV family genes have been performed in several species, little is known about the family in soybean (Glycine max L.). In this study, a total of 13 RAV genes, named as GmRAVs, were identified in the soybean genome. We predicted and analyzed the amino acid compositions, phylogenetic relationships, and folding states of conserved domain sequences of soybean RAV transcription factors. These soybean RAV transcription factors were phylogenetically clustered into three classes based on their amino acid sequences. Subcellular localization analysis revealed that the soybean RAV proteins were located in the nucleus. The expression patterns of 13 RAV genes were analyzed by quantitative real-time PCR. Under drought stresses, the RAV genes expressed diversely, up-or down-regulated. Following NaCl treatments, all RAV genes were down-regulated excepting GmRAV-03 which was up-regulated. Under abscisic acid (ABA) treatment, the expression of all of the soybean RAV genes increased dramatically. These results suggested that the soybean RAV genes may be involved in diverse signaling pathways and may be responsive to abiotic stresses and exogenous ABA. Further analysis indicated that GmRAV-03 could increase the transgenic lines resistance to high salt and drought and result in the transgenic plants insensitive to exogenous ABA. This present study provides valuable information for understanding the classification and putative functions of the RAV transcription factors in soybean.
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Claims 10
Claim · model-written summary
Thirteen GmRAV genes were identified in the soybean genome.
- Taxon
- soybean
Glycine maxe0 - Gene
- GmRAVse0
- Outcome
- RAV genese0
- Value
- 13 RAV genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 13 RAV genes, named as GmRAVs, were identified in the soybean genome”
Claim · model-written summary
Soybean RAV transcription factors clustered into three phylogenetic classes.
- Taxon
- soybeanGlycine maxe0
- Gene
- RAV transcription factorse0
- Outcome
- phylogenetically clustered into three classese0
- Value
- three classese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean RAV transcription factors were phylogenetically clustered into three classes based on their amino acid sequences”
Claim · model-written summary
Soybean RAV proteins were located in the nucleus.
- Taxon
- soybeanGlycine maxe0
- Gene
- RAV proteinse0
- Outcome
- located in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean RAV proteins were located in the nucleus”
Claim · model-written summary
Drought stress caused diverse up- or down-regulation of RAV genes.
- Taxon
- soybeanGlycine maxe1
- Treatment
- drought stressese0
- Gene
- RAV genese0
- Outcome
- RAV genes expressede0
- Direction
- up-or down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought stresses, the RAV genes expressed diversely, up-or down-regulated”
e1“These soybean RAV transcription factors were phylogenetically clustered into three classes”
Claim · model-written summary
NaCl down-regulated all RAV genes except GmRAV-03.
- Taxon
- soybeanGlycine maxe1
- Treatment
- NaCl treatmentse0
- Gene
- RAV genese0
- Outcome
- RAV genese0
- Direction
- down-regulatede0
- Qualifier
- excepting GmRAV-03e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Following NaCl treatments, all RAV genes were down-regulated excepting GmRAV-03 which was up-regulated”
e1“These soybean RAV transcription factors were phylogenetically clustered into three classes”
Claim · model-written summary
NaCl up-regulated GmRAV-03.
- Taxon
- soybeanGlycine maxe1
- Treatment
- NaCl treatmentse0
- Gene
- GmRAV-03e0
- Outcome
- GmRAV-03e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Following NaCl treatments, all RAV genes were down-regulated excepting GmRAV-03 which was up-regulated”
e1“These soybean RAV transcription factors were phylogenetically clustered into three classes”
Claim · model-written summary
ABA treatment dramatically increased expression of all soybean RAV genes.
- Taxon
- soybeanGlycine maxe0
- Treatment
- abscisic acid (ABA) treatmente0
- Gene
- RAV genese0
- Outcome
- expression of all of the soybean RAV genese0
- Direction
- increased dramaticallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under abscisic acid (ABA) treatment, the expression of all of the soybean RAV genes increased dramatically”
Claim · model-written summary
GmRAV-03 increased transgenic line resistance to high salt.
- Taxon
- soybeanGlycine maxe1
- Material
- transgenic linese0
- Treatment
- GmRAV-03e0
- Gene
- GmRAV-03e0
- Outcome
- resistance to high salte0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmRAV-03 could increase the transgenic lines resistance to high salt and drought”
e1“These soybean RAV transcription factors were phylogenetically clustered into three classes”
Claim · model-written summary
GmRAV-03 increased transgenic line resistance to drought.
- Taxon
- soybeanGlycine maxe1
- Material
- transgenic linese0
- Treatment
- GmRAV-03e0
- Gene
- GmRAV-03e0
- Outcome
- resistance to high salt and droughte0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmRAV-03 could increase the transgenic lines resistance to high salt and drought”
e1“These soybean RAV transcription factors were phylogenetically clustered into three classes”
Claim · model-written summary
GmRAV-03 made transgenic plants insensitive to exogenous ABA.
- Taxon
- soybeanGlycine maxe1
- Treatment
- GmRAV-03e0
- Gene
- GmRAV-03e0
- Outcome
- transgenic plants insensitive to exogenous ABAe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmRAV-03 could increase the transgenic lines resistance to high salt and drought and result in the transgenic plants insensitive to exogenous ABA”
e1“These soybean RAV transcription factors were phylogenetically clustered into three classes”
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