Abstract
The CAPRICE (CPC) gene encodes a R3-type MYB transcription factor that promotes differentiation of root hair cells in Arabidopsis thaliana. Here, we have compared the functions of five CPC-homologous genes for epidermal cell differentiation using CPC promoter-driven transgenic plants. Our results show that TRIPTYCHON (TRY) and ENHANCER OF TRY AND CPC2 (ETC2) were less effective in root hair cell differentiation and were unstable in root epidermal cells when compared with CPC, ETC1 or CPC LIKE MYB3 (CPL3). The deletion of the extended C-terminal domain of TRY and ETC2 enhanced protein stability and conferred the ability to induce root hair cell differentiation on them. Treatment with MG132, a proteasome inhibitor, also led to the accumulation of TRY, indicating that TRY proteolysis is mediated by the proteasome-dependent pathway. Our results indicate that the CPC family includes relatively stable (CPC, ETC1 and CPL3) and unstable (TRY and ETC2) proteins that might be degraded by the proteasome. Our findings provide new insights into the regulatory mechanism of CPC family proteins that mediate root hair cell differentiation and should be useful in understanding epidermal development.
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Claims 5
Claim · model-written summary
TRY and ETC2 were less effective at root hair differentiation than CPC, ETC1, or CPL3.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Gene
- TRIPTYCHON (TRY) and ENHANCER OF TRY AND CPC2 (ETC2)e0
- Outcome
- root hair cell differentiatione0
- Direction
- less effectivee0
- Comparator
- CPC, ETC1 or CPC LIKE MYB3 (CPL3)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TRIPTYCHON (TRY) and ENHANCER OF TRY AND CPC2 (ETC2) were less effective in root hair cell differentiation and were unstable in root epidermal cells when compared with CPC, ETC1 or CPC LIKE MYB3 (CPL3).”
e1“Arabidopsis thaliana”
Claim · model-written summary
TRY and ETC2 were unstable in root epidermal cells relative to CPC, ETC1, and CPL3.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- root epidermal cellse0
- Gene
- TRIPTYCHON (TRY) and ENHANCER OF TRY AND CPC2 (ETC2)e0
- Outcome
- unstable in root epidermal cellse0
- Comparator
- CPC, ETC1 or CPC LIKE MYB3 (CPL3)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TRIPTYCHON (TRY) and ENHANCER OF TRY AND CPC2 (ETC2) were less effective in root hair cell differentiation and were unstable in root epidermal cells when compared with CPC, ETC1 or CPC LIKE MYB3 (CPL3).”
e1“Arabidopsis thaliana”
Claim · model-written summary
Deleting the extended C-terminal domain enhanced TRY and ETC2 protein stability.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- deletion of the extended C-terminal domaine0
- Gene
- TRY and ETC2e0
- Outcome
- protein stabilitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deletion of the extended C-terminal domain of TRY and ETC2 enhanced protein stability”
e1“Arabidopsis thaliana”
Claim · model-written summary
Deleting the extended C-terminal domain gave TRY and ETC2 the ability to induce root hair differentiation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- deletion of the extended C-terminal domaine0
- Gene
- TRY and ETC2e0
- Outcome
- ability to induce root hair cell differentiatione0
- Direction
- conferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deletion of the extended C-terminal domain of TRY and ETC2 enhanced protein stability and conferred the ability to induce root hair cell differentiation on them.”
e1“Arabidopsis thaliana”
Claim · model-written summary
MG132 treatment led to TRY accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- MG132e0
- Gene
- TRYe0
- Outcome
- accumulation of TRYe0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with MG132, a proteasome inhibitor, also led to the accumulation of TRY”
e1“Arabidopsis thaliana”
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