Abstract
The present investigation on effect of different growth regulator combinations on the per cent media browning in walnut in vitro studies using MS medium was carried out in order to document the available genetic variability in walnut germplasm and to select elite walnut genotypes possessing superior attributes and quality traits.During the survey, data was recorded on one hundred fifty two (152) walnut trees growing in different areas of Kashmir valley.The study also involved establishment of response of elite walnut selections to different plant growth regulators in shoot morphogenesis.Woody species have been found to be far more difficult to clone in vitro than herbaceous plants.Poor response of the explants from mature woody species to in vitro manipulation is usually associated with the problem of browning and explant necrosis.The present studies were conducted on forced explants from three walnut selections (SKUAST 002, SKUAST 008, SKUAST 010).Murashiage and Skoog's basal medium supplemented with 0.3 mg/l -1 Benzylamino purine and 0.1 mg/l -1 indole-3-butyric acid gave best response in the establishment of initiating cultures, minimum media browning (80.44 %), minimum explant browning (78.22 %) and minimum mean browning score per explant (9.17 %).The survival (23.45%) and growth of the cultures (21.77%) was also found to be maximum in MS medium supplemented with BAP 0.3 mg l -1 and IBA 0.1 mg l -1 .
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Claims 6
Claim · model-written summary
MS medium with 0.3 mg/l Benzylamino purine and 0.1 mg/l indole-3-butyric acid gave the best initiating-culture establishment.
- Taxon
- walnutt0
- Treatment
- Murashiage and Skoog's basal medium supplemented with 0.3 mg/l -1 Benzylamino purine and 0.1 mg/l -1 indole-3-butyric acide0
- Outcome
- establishment of initiating culturese0
- Direction
- best responsee0
Not reportedComparator
Verbatim
e0“Murashiage and Skoog's basal medium supplemented with 0.3 mg/l -1 Benzylamino purine and 0.1 mg/l -1 indole-3-butyric acid gave best response in the establishment of initiating cultures”
t0“walnut”
Claim · model-written summary
That medium yielded minimum media browning, reported as 80.44%.
Not reportedTreatmentComparator
Verbatim
e0“minimum media browning (80.44 %)”
t0“walnut”
Claim · model-written summary
That medium yielded minimum explant browning, reported as 78.22%.
Not reportedTreatmentComparator
Verbatim
e0“minimum explant browning (78.22 %)”
t0“walnut”
Claim · model-written summary
That medium yielded a minimum mean browning score per explant of 9.17%.
Not reportedTreatmentComparator
Verbatim
e0“minimum mean browning score per explant (9.17 %)”
t0“walnut”
Claim · model-written summary
MS medium with BAP 0.3 mg l -1 and IBA 0.1 mg l -1 maximized culture survival at 23.45%.
- Taxon
- walnutt0
- Treatment
- MS medium supplemented with BAP 0.3 mg l -1 and IBA 0.1 mg l -1e0
- Outcome
- survivale0
- Direction
- maximume0
- Value
- 23.45%e0
Not reportedComparator
Verbatim
e0“The survival (23.45%) and growth of the cultures (21.77%) was also found to be maximum in MS medium supplemented with BAP 0.3 mg l -1 and IBA 0.1 mg l -1”
t0“walnut”
Claim · model-written summary
MS medium with BAP 0.3 mg l -1 and IBA 0.1 mg l -1 maximized culture growth at 21.77%.
- Taxon
- walnutt0
- Treatment
- MS medium supplemented with BAP 0.3 mg l -1 and IBA 0.1 mg l -1e0
- Outcome
- growth of the culturese0
- Direction
- maximume0
- Value
- 21.77%e0
Not reportedComparator
Verbatim
e0“The survival (23.45%) and growth of the cultures (21.77%) was also found to be maximum in MS medium supplemented with BAP 0.3 mg l -1 and IBA 0.1 mg l -1”
t0“walnut”
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