Abstract
Agrobacterium-mediated transformation is an important research tool for the genetic improvement of cassava. The induction of friable embryogenic callus (FEC) is considered as a key step in cassava transformation. In the present study, the media composition was optimized for enhancing the FEC induction, and the effect of the optimized medium on gene expression was evaluated. In relative comparison to MS medium, results demonstrated that using a medium with reducing nutrition (a 10-fold less concentration of nitrogen, potassium, and phosphate), the increased amount of vitamin B1 (10 mg/L) and the use of picrolam led to reprogram non-FEC to FEC. Gene expression analyses revealed that FEC on modified media increased the expression of genes related to the regulation of polysaccharide biosynthesis and breakdown of cell wall components in comparison to FEC on normal CIM media, whereas the gene expression associated with energy flux was not dramatically altered. It is hypothesized that we reprogram non-FEC to FEC under low nitrogen, potassium and phosphate and high vitamin B1. These findings were more effective in inducing FEC formation than the previous protocol. It might contribute to development of an efficient transformation strategy in cassava.
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Claims 4
Claim · model-written summary
Reduced nutrients, increased vitamin B1 and picrolam induced conversion of non-FEC to FEC.
- Taxon
- cassava
Manihot esculentat0 - Treatment
- a medium with reducing nutrition (a 10-fold less concentration of nitrogen, potassium, and phosphate), the increased amount of vitamin B1 (10 mg/L) and the use of picrolame0
- Outcome
- reprogram non-FEC to FECe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using a medium with reducing nutrition (a 10-fold less concentration of nitrogen, potassium, and phosphate), the increased amount of vitamin B1 (10 mg/L) and the use of picrolam led to reprogram non-FEC to FEC.”
t0“cassava”
Claim · model-written summary
FEC on modified media showed increased expression of cell wall polysaccharide metabolism genes relative to normal CIM.
- Taxon
- cassavaManihot esculentat0
- Treatment
- modified mediae0
- Outcome
- expression of genes related to the regulation of polysaccharide biosynthesis and breakdown of cell wall componentse0
- Direction
- increasede0
- Comparator
- normal CIM mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FEC on modified media increased the expression of genes related to the regulation of polysaccharide biosynthesis and breakdown of cell wall components in comparison to FEC on normal CIM media”
t0“cassava”
Claim · model-written summary
Expression of energy flux genes was not dramatically altered on modified media.
- Taxon
- cassavaManihot esculentat0
- Treatment
- modified mediae1
- Outcome
- gene expression associated with energy fluxe0
- Direction
- not dramatically alterede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the gene expression associated with energy flux was not dramatically altered.”
t0“cassava”
e1“modified media”
Claim · model-written summary
The modified medium induced FEC formation more effectively than the previous protocol.
- Taxon
- cassavaManihot esculentat0
- Outcome
- inducing FEC formatione0
- Direction
- more effectivee0
- Comparator
- the previous protocole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These findings were more effective in inducing FEC formation than the previous protocol.”
t0“cassava”
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