Abstract
Abstract: The effect of sucrose with or without abscisic acid (ABA) in cryoprotective medium on post-thaw embryogenic competence and subsequent plant recovery of embryogenic callus clumps of cassava was investigated. Post-thaw viability depended on duration of cryoprotection on the 0.3M sucrose cryoprotective medium. The presence of only ABA in the cryoprotective medium resulted in callus proliferation but loss of post-thaw viability. However, the inclusion of sucrose with low concentrations of ABA (5-10 mg/l) in the cryoprotective medium decreased callus proliferation but subsequent cryopreservation resulted in post-thaw viability and/or embryogenic competence, except at the higher concentration of ABA (20mg/l) where there was complete loss of post-thaw viability. Also, callus clumps pre-treated with low concentrations of ABA with sucrose prior to cryopreservation resulted in significantly higher plant recovery than only sucrose pre-treated callus clumps. Plants recovered from cryopreserved callus clumps were phenotypically similar to non-cryopreserved callus clumps. These results indicates that cryopreservation of embryogenic calli of cassava is a feasible option for long-term conservation of the crops ’ genetic resources. Key words: Abscisic acid • Cryopreservation • Embryogenic calli • Embryogenic competence
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Claims 9
Claim · model-written summary
Post-thaw viability of cassava callus clumps depended on the duration of cryoprotection on 0.3M sucrose.
- Taxon
- Cassava
Manihot esculentat0 - Treatment
- duration of cryoprotectione1
- Outcome
- Post-thaw viabilitye1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cassava”
e1“Post-thaw viability depended on duration of cryoprotection on the 0.3M sucrose cryoprotective medium”
Claim · model-written summary
Cassava callus in a cryoprotective medium containing only ABA increased callus proliferation.
- Taxon
- CassavaManihot esculentat0
- Treatment
- only ABA in the cryoprotective mediume2
- Outcome
- callus proliferatione2
- Direction
- increaseinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cassava”
e2“The presence of only ABA in the cryoprotective medium resulted in callus proliferation but loss of post-thaw viability”
Claim · model-written summary
Cassava callus in a cryoprotective medium containing only ABA decreased post-thaw viability.
- Taxon
- CassavaManihot esculentat0
- Treatment
- only ABA in the cryoprotective mediume2
- Outcome
- post-thaw viabilitye2
- Direction
- loss ofe2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cassava”
e2“The presence of only ABA in the cryoprotective medium resulted in callus proliferation but loss of post-thaw viability”
Claim · model-written summary
Sucrose with low ABA concentrations (5-10 mg/l) in the cryoprotective medium decreased callus proliferation.
- Taxon
- CassavaManihot esculentat0
- Treatment
- the inclusion of sucrose with low concentrations of ABA (5-10 mg/l)e3
- Outcome
- callus proliferatione3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cassava”
e3“the inclusion of sucrose with low concentrations of ABA (5-10 mg/l) in the cryoprotective medium decreased callus proliferation”
Claim · model-written summary
Sucrose with low ABA concentrations (5-10 mg/l) in the cryoprotective medium resulted in post-thaw viability and/or embryogenic competence after cryopreservation.
- Taxon
- CassavaManihot esculentat0
- Treatment
- the inclusion of sucrose with low concentrations of ABA (5-10 mg/l)e5
- Outcome
- post-thaw viability and/or embryogenic competencee5
- Direction
- resulted ine5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cassava”
e5“the inclusion of sucrose with low concentrations of ABA (5-10 mg/l) in the cryoprotective medium decreased callus proliferation but subsequent cryopreservation resulted in post-thaw viability and/or embryogenic competence”
Claim · model-written summary
At the higher ABA concentration of 20 mg/l, post-thaw viability of cassava callus was completely lost.
- Taxon
- CassavaManihot esculentat0
- Treatment
- higher concentration of ABA (20mg/l)e6
- Outcome
- post-thaw viabilitye6
- Direction
- complete losse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cassava”
e6“except at the higher concentration of ABA (20mg/l) where there was complete loss of post-thaw viability”
Claim · model-written summary
Cassava callus clumps pre-treated with low ABA concentrations with sucrose gave significantly higher plant recovery than sucrose-only pre-treated clumps.
- Taxon
- CassavaManihot esculentat0
- Treatment
- pre-treated with low concentrations of ABA with sucrosee7
- Outcome
- plant recoverye7
- Direction
- significantly highere7
- Comparator
- only sucrose pre-treated callus clumpse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cassava”
e7“callus clumps pre-treated with low concentrations of ABA with sucrose prior to cryopreservation resulted in significantly higher plant recovery than only sucrose pre-treated callus clumps”
Claim · model-written summary
Plants recovered from cryopreserved cassava callus clumps were phenotypically similar to those from non-cryopreserved clumps.
- Taxon
- CassavaManihot esculentat0
- Treatment
- cryopreserved callus clumpse7
- Outcome
- Plants recoverede7
- Direction
- phenotypically similare7
- Comparator
- non-cryopreserved callus clumpse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cassava”
e7“Plants recovered from cryopreserved callus clumps were phenotypically similar to non-cryopreserved callus clumps”
Claim · model-written summary
Cryopreservation of cassava embryogenic calli is a feasible option for long-term conservation of crop genetic resources.
- Taxon
- CassavaManihot esculentat0
- Outcome
- feasible option for long-term conservatione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cassava”
e7“cryopreservation of embryogenic calli of cassava is a feasible option for long-term conservation of the crops ’ genetic resources”
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See all 35 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.