Abstract
CBF (C-repeat binding factor) transcription factors show high expression levels in response to cold; moreover, they play a key regulatory role in cold acclimation processes. Recently, however, more and more information has led to the conclusion that, apart from cold, light—including its spectra—also has a crucial role in regulating CBF expression. Earlier, studies established that the expression patterns of some of these regulatory genes follow circadian rhythms. To understand more of this complex acclimation process, we studied the expression patterns of the signal transducing pathways, including signal perception, the circadian clock and phospholipid signalling pathways, upstream of the CBF gene regulatory hub. To exclude the confounding effect of cold, experiments were carried out at 22 °C. Our results show that the expression of genes implicated in the phospholipid signalling pathway follow a circadian rhythm. We demonstrated that, from among the tested CBF genes expressed in Hordeum vulgare (Hv) under our conditions, only the members of the HvCBF4-phylogenetic subgroup showed a circadian pattern. We found that the HvCBF4-subgroup genes were expressed late in the afternoon or early in the night. We also determined the expression changes under supplemental far-red illumination and established that the transcript accumulation had appeared four hours earlier and more intensely in several cases. Based on our results, we propose a model to illustrate the effect of the circadian clock and the quality of the light on the elements of signalling pathways upstream of the HvCBFs, thus integrating the complex regulation of the early cellular responses, which finally lead to an elevated abiotic stress tolerance.
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Claims 4
Claim · model-written summary
Phospholipid signaling genes followed a circadian rhythm.
- Taxon
- Barley
.jpg?width=60)
Hordeum vulgaret0 - Outcome
- expression of genes implicated in the phospholipid signalling pathwaye0
- Qualifier
- circadian rhythme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“the expression of genes implicated in the phospholipid signalling pathway follow a circadian rhythm”
t0“Barley”
Claim · model-written summary
Only the HvCBF4 phylogenetic subgroup among tested CBF genes showed a circadian pattern.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- HvCBF4-phylogenetic subgroupe0
- Outcome
- circadian patterne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only the members of the HvCBF4-phylogenetic subgroup showed a circadian pattern”
t0“Barley”
Claim · model-written summary
HvCBF4 subgroup genes were expressed late in the afternoon or early at night.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- HvCBF4-subgroup genese0
- Outcome
- expressed late in the afternoon or early in the nighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the HvCBF4-subgroup genes were expressed late in the afternoon or early in the night”
t0“Barley”
Claim · model-written summary
Supplemental far-red illumination advanced and intensified transcript accumulation in several cases.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- supplemental far-red illuminatione0
- Outcome
- transcript accumulatione0
- Direction
- earliere0
- Value
- four hourse0
- Qualifier
- in several casese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under supplemental far-red illumination and established that the transcript accumulation had appeared four hours earlier and more intensely in several cases”
t0“Barley”
The same questions in other papers
light · molecular: expression · Hordeum vulgare (barley)Positive and negative results
19 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- no effect
20 papers more in this group
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