Abstract
Sugarcane has a paramount importance among the cash crop in India. Commercially sugarcane is propagated by stem cutting with each cutting or set having two or three buds. After the development of clone or variety, major bottleneck in spreading of clone or variety is slow propagation rate through conventional method, which takes years. Micropropagation is only a realistic means of achieving rapid, large scale production of disease free seed cane of newly developed varieties in order to speeding and commercialization process in sugarcane. Standardization of protocol for induction of callus and shoot regeneration was established through in vitro culture of sugarcane. The best callus induction was observed at 4 mg/l of 2, 4-D. The best response in terms of multiple shoot formation was observed on MS medium supplemented with 0.5 mg/l BAP and 1 mg/l NAA. Best rooting was observed in half-strength MS medium + 2% sucrose supplemented with 0.5 mg/l NAA and 1 mg/l IBA among different auxin. Best hardening response was observed in potting mixture containing Clay + Sand + Bio-compost (1: 1: 1 ratio) in primary and secondary hardening.
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Claims 4
Claim · model-written summary
Sugarcane had the best callus induction with 4 mg/l of 2, 4-D.
Not reportedComparator
Verbatim
e0“The best callus induction was observed at 4 mg/l of 2, 4-D.”
t0“Sugarcane”
Claim · model-written summary
Sugarcane had the best multiple shoot formation with MS medium supplemented with 0.5 mg/l BAP and 1 mg/l NAA.
- Taxon
- Sugarcanet0
- Treatment
- MS medium supplemented with 0.5 mg/l BAP and 1 mg/l NAAe0
- Outcome
- multiple shoot formatione0
- Direction
- beste0
Not reportedComparator
Verbatim
e0“The best response in terms of multiple shoot formation was observed on MS medium supplemented with 0.5 mg/l BAP and 1 mg/l NAA.”
t0“Sugarcane”
Claim · model-written summary
Sugarcane had the best rooting with half-strength MS medium + 2% sucrose supplemented with 0.5 mg/l NAA and 1 mg/l IBA.
- Taxon
- Sugarcanet0
- Treatment
- half-strength MS medium + 2% sucrose supplemented with 0.5 mg/l NAA and 1 mg/l IBAe0
- Outcome
- rootinge0
- Direction
- Beste0
Not reportedComparator
Verbatim
e0“Best rooting was observed in half-strength MS medium + 2% sucrose supplemented with 0.5 mg/l NAA and 1 mg/l IBA among different auxin.”
t0“Sugarcane”
Claim · model-written summary
Sugarcane had the best hardening response with potting mixture containing Clay + Sand + Bio-compost (1: 1: 1 ratio).
- Taxon
- Sugarcanet0
- Treatment
- potting mixture containing Clay + Sand + Bio-compost (1: 1: 1 ratio)e0
- Outcome
- hardening responsee0
- Direction
- Beste0
Not reportedComparator
Verbatim
e0“Best hardening response was observed in potting mixture containing Clay + Sand + Bio-compost (1: 1: 1 ratio) in primary and secondary hardening.”
t0“Sugarcane”
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