Abstract
Ascorbic acid (AsA) is one of the important metabolites and beneficial to human health. First of all, we found the phenomenon that light could promote AsA accumulation in the peel of eggplant. To further understand the regulatory mechanism of light on the formation of AsA in fruits, we isolated seven novel L-galactose pathway related genes (SmGMP, SmGME1, SmGME2, SmGGP, SmGPP, SmGalDH and SmGLDH) in AsA biosynthesis from eggplant. Correlation analysis suggested that AsA content showed significant positive correlation with the expression levels of SmGME2, SmGGP, SmGPP, SmGalDH and SmGLDH. There were many putative light responsive elements in all the seven promoters of genes, and the expression analysis confirmed that the seven genes were induced by light. Then we overexpressed the seven genes in Arabidopsis and observed that the AsA content were significantly increased in the leaves of transgenic plants. Moreover, the transgenic plants also displayed an increase in low temperature tolerance. The present study provides insight into light regulation of AsA biosynthesis in eggplant and may facilitate genetic engineering for improvement of the AsA content and stress tolerance in plants.
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Claims 7
Claim · model-written summary
Light promoted ascorbic acid accumulation in eggplant peel.
- Taxon
- eggplant
Solanum melongenae0 - Organ / stage
- peele0
- Treatment
- lighte0
- Outcome
- AsA accumulatione0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“light could promote AsA accumulation in the peel of eggplant”
Claim · model-written summary
Seven L-galactose pathway genes involved in ascorbic acid biosynthesis were isolated from eggplant.
- Taxon
- eggplantSolanum melongenae0
- Gene
- SmGMP, SmGME1, SmGME2, SmGGP, SmGPP, SmGalDH and SmGLDHe0
- Outcome
- seven novel L-galactose pathway related genese0
- Value
- sevene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated seven novel L-galactose pathway related genes (SmGMP, SmGME1, SmGME2, SmGGP, SmGPP, SmGalDH and SmGLDH) in AsA biosynthesis from eggplant”
Claim · model-written summary
Ascorbic acid content was significantly positively correlated with expression of five pathway genes.
- Taxon
- eggplantSolanum melongenat0
- Gene
- SmGME2, SmGGP, SmGPP, SmGalDH and SmGLDHe0
- Outcome
- AsA contente0
- Direction
- positive correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AsA content showed significant positive correlation with the expression levels of SmGME2, SmGGP, SmGPP, SmGalDH and SmGLDH”
t0“eggplant”
Claim · model-written summary
Putative light responsive elements occurred in the promoters of all seven genes.
- Taxon
- eggplantSolanum melongenat0
- Outcome
- putative light responsive elementse0
- Qualifier
- all the seven promoters of genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were many putative light responsive elements in all the seven promoters of genes”
t0“eggplant”
Claim · model-written summary
Light induced expression of the seven eggplant pathway genes.
- Taxon
- eggplantSolanum melongenat0
- Treatment
- lighte0
- Outcome
- the seven genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the seven genes were induced by light”
t0“eggplant”
Claim · model-written summary
Overexpression of the seven genes significantly increased leaf ascorbic acid content in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- leavese0
- Treatment
- overexpressed the seven genese0
- Outcome
- AsA contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we overexpressed the seven genes in Arabidopsis and observed that the AsA content were significantly increased in the leaves of transgenic plants”
Claim · model-written summary
The transgenic Arabidopsis plants also had increased low temperature tolerance.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- transgenic plantse0
- Outcome
- low temperature tolerancee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenic plants also displayed an increase in low temperature tolerance”
e1“in Arabidopsis”
The same questions in other papers
light · molecular: metabolites · Solanum melongena (eggplant)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.