Abstract
Coniothyrium minitans is an important mycoparasite of Sclerotinia sclerotiorum. In addition, it also produces small amounts of antifungal substances. ZS-1TN1812, an abnormal mutant, was originally screened from a T-DNA insertional library. This mutant showed abnormal growth phenotype and could significantly inhibit the growth of S. sclerotiorum when dual-cultured on a PDA plate. When spraying the filtrate of ZS-1TN1812 on the leaves of rapeseed, S. sclerotiorum infection was significantly inhibited, suggesting that the antifungal substances produced by this mutant were effective on rapeseed leaves. The thermo-tolerant antifungal substances could specifically suppress the growth of S. sclerotiorum, but could not significantly suppress the growth of another fungus, Colletotrichum higginsianum. However, C. higginsianum was more sensitive to proteinous antibiotics than S. sclerotiorum. The T-DNA insertion in ZS-1TN1812 activated the expression of CmSIT1, a gene involved in siderophore-mediated iron transport. It was also determined that mutant ZS-1TN1812 produced hypha with high iron levels. In the wild-type strain, ZS-1, CmSIT1 was expressed only when in contact with S. sclerotiorum, and consistent overexpression of CmSIT1 showed similar phenotypes in ZS-1TN1812. Therefore, activated expression of CmSIT1 leads to the enhanced antifungal ability, and CmSIT1 is a potential gene for improving the control of C. minitans.
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Claims 11
Claim · model-written summary
The ZS-1TN1812 mutant had an abnormal growth phenotype.
Verbatim
e0“This mutant showed abnormal growth phenotype and could significantly inhibit the growth of S. sclerotiorum when dual-cultured on a PDA plate.”
e7“leaves of rapeseed”
Claim · model-written summary
The mutant inhibited S. sclerotiorum growth in dual culture.
- Taxon
- rapeseede7
- Treatment
- This mutante0
- Interactor
- S. sclerotiorum
Sclerotinia sclerotiorume0pathogen - Outcome
- growth of S. sclerotiorume0
- Direction
- inhibite0
- Setting
- PDA platee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This mutant showed abnormal growth phenotype and could significantly inhibit the growth of S. sclerotiorum when dual-cultured on a PDA plate.”
e7“leaves of rapeseed”
Claim · model-written summary
Spraying ZS-1TN1812 filtrate significantly inhibited S. sclerotiorum infection of rapeseed leaves.
- Taxon
- rapeseede0
- Organ / stage
- leavese0
- Treatment
- spraying the filtrate of ZS-1TN1812e0
- Interactor
- S. sclerotiorumSclerotinia sclerotiorume0pathogen
- Outcome
- S. sclerotiorum infectione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When spraying the filtrate of ZS-1TN1812 on the leaves of rapeseed, S. sclerotiorum infection was significantly inhibited”
Claim · model-written summary
Thermotolerant antifungal substances suppressed S. sclerotiorum growth.
- Taxon
- rapeseede7
- Treatment
- thermo-tolerant antifungal substancese0
- Interactor
- S. sclerotiorumSclerotinia sclerotiorume0pathogen
- Outcome
- growth of S. sclerotiorume0
- Direction
- suppresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The thermo-tolerant antifungal substances could specifically suppress the growth of S. sclerotiorum”
e7“leaves of rapeseed”
Claim · model-written summary
The substances did not significantly suppress C. higginsianum growth.
- Taxon
- rapeseede7
- Interactor
- Colletotrichum higginsianumColletotrichum higginsianume0pathogen
- Outcome
- growth of another fungus, Colletotrichum higginsianume0
- Direction
- could not significantly suppresse0
Not reportedTreatmentComparator
Verbatim
e0“could not significantly suppress the growth of another fungus, Colletotrichum higginsianum.”
e7“leaves of rapeseed”
Claim · model-written summary
C. higginsianum was more sensitive to proteinous antibiotics than S. sclerotiorum.
Verbatim
e0“C. higginsianum was more sensitive to proteinous antibiotics than S. sclerotiorum.”
e7“leaves of rapeseed”
Claim · model-written summary
T-DNA insertion activated CmSIT1 expression in ZS-1TN1812.
Verbatim
e0“The T-DNA insertion in ZS-1TN1812 activated the expression of CmSIT1”
e7“leaves of rapeseed”
Claim · model-written summary
ZS-1TN1812 hyphae had high iron levels.
Verbatim
e0“mutant ZS-1TN1812 produced hypha with high iron levels.”
e7“leaves of rapeseed”
Claim · model-written summary
Wild-type ZS-1 expressed CmSIT1 only in contact with S. sclerotiorum.
- Taxon
- rapeseede7
- Material
- ZS-1e0
- Interactor
- S. sclerotiorumSclerotinia sclerotiorume0pathogen
- Gene
- CmSIT1e0
- Outcome
- CmSIT1 was expressede0
- Qualifier
- only when in contact with S. sclerotiorume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the wild-type strain, ZS-1, CmSIT1 was expressed only when in contact with S. sclerotiorum”
e7“leaves of rapeseed”
Claim · model-written summary
Consistent CmSIT1 overexpression reproduced ZS-1TN1812 phenotypes.
- Taxon
- rapeseede7
- Treatment
- consistent overexpression of CmSIT1e0
- Gene
- CmSIT1e0
- Outcome
- similar phenotypese0
- Comparator
- ZS-1TN1812e0
Not reportedDirection
Verbatim
e0“consistent overexpression of CmSIT1 showed similar phenotypes in ZS-1TN1812.”
e7“leaves of rapeseed”
Claim · model-written summary
The authors propose CmSIT1 as a gene to improve C. minitans control.
Verbatim
e0“CmSIT1 is a potential gene for improving the control of C. minitans.”
e7“leaves of rapeseed”
The same questions in other papers
antifungal agent · Sclerotinia sclerotiorum · Brassica napus (oilseed rape)Results agree
1 paper in the same direction as this paper
- positive
application · Sclerotinia sclerotiorum · Brassica napus (oilseed rape)Positive and negative results
8 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
- negative
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.