Abstract
Dehydrins (DHNs) are intrinsically disordered proteins that play central roles in plant abiotic stress responses; however, how they work remains unclear. Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions. To explore the dimerization ability of the Arabidopsis acidic DHNs AtCOR47 and AtERD10, we conducted an in planta DHN binding assay using the Bimolecular Fluorescence Complementation (BiFC) technique. Our analyses revealed homodimeric interactions for AtCOR47 and AtERD10; interestingly, heterodimeric associations also occurred with these DHNs, and these interactions were observed in the cytosol of tobacco cells. Furthermore, we evaluated whether Arabidopsis basic DHNs, such as AtRAB18, could also interact with itself and/or with AtCOR47 and AtERD10 in the BiFC system. Our data revealed homodimeric RAB18 complexes in the nucleus and cytosol, while heterodimeric associations between AtRAB18 and acidic DHNs occurred only in the cytosol. Finally, we demonstrated the presence of heterodimeric complexes among Arabidopsis AtCOR47, AtERD10, and AtRAB18 DHNs with their acidic ortholog the OpsDHN1 from Opuntia streptacantha; these heterodimeric interactions showed different subcellular distributions. Our results guide DHN research toward a new scenario where DHN/DHN oligomerization could be explored as a part of their molecular mechanism.
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Claims 8
Claim · model-written summary
Arabidopsis dehydrins AtCOR47, AtERD10 and AtRAB18 were localized in planta with GFP fusions
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Gene
- AtCOR47, AtERD10, and AtRAB18e1
- Outcome
- subcellular localizatione1
- Qualifier
- through GFP translational fusionse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions”
Claim · model-written summary
AtCOR47 and AtERD10 formed homodimers in planta
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- AtCOR47 and AtERD10e2
- Outcome
- homodimeric interactionse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions”
e2“Our analyses revealed homodimeric interactions for AtCOR47 and AtERD10”
Claim · model-written summary
AtCOR47 and AtERD10 formed heterodimeric associations observed in the cytosol of tobacco cells
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- AtCOR47 and AtERD10e2
- Outcome
- heterodimeric associationse3
- Qualifier
- observed in the cytosol of tobacco cellse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions”
e2“Our analyses revealed homodimeric interactions for AtCOR47 and AtERD10”
e3“interestingly, heterodimeric associations also occurred with these DHNs, and these interactions were observed in the cytosol of tobacco cells”
Claim · model-written summary
AtRAB18 formed homodimeric complexes in the nucleus and cytosol
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- RAB18e4
- Outcome
- homodimeric RAB18 complexese4
- Qualifier
- in the nucleus and cytosole4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions”
e4“Our data revealed homodimeric RAB18 complexes in the nucleus and cytosol”
Claim · model-written summary
Heterodimeric associations between AtRAB18 and acidic dehydrins occurred only in the cytosol
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- AtRAB18e5
- Outcome
- heterodimeric associationse5
- Qualifier
- occurred only in the cytosole5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions”
e5“heterodimeric associations between AtRAB18 and acidic DHNs occurred only in the cytosol”
Claim · model-written summary
Heterodimeric complexes formed among AtCOR47, AtERD10 and AtRAB18 with the acidic OpsDHN1 ortholog from Opuntia streptacantha
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Interactor
- Opuntia streptacantha
Opuntia streptacanthae6other - Gene
- AtCOR47, AtERD10, and AtRAB18e6
- Outcome
- presence of heterodimeric complexese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions”
e6“the presence of heterodimeric complexes among Arabidopsis AtCOR47, AtERD10, and AtRAB18 DHNs with their acidic ortholog the OpsDHN1 from Opuntia streptacantha”
Claim · model-written summary
The heterodimeric interactions showed different subcellular distributions
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- subcellular distributionse7
- Qualifier
- showed differente7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions”
e7“these heterodimeric interactions showed different subcellular distributions”
Claim · model-written summary
DHN/DHN oligomerization could be part of the molecular mechanism of dehydrins
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- DHN/DHN oligomerizatione2
- Qualifier
- could be explored as a part of their molecular mechanisme2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions”
e2“Our results guide DHN research toward a new scenario where DHN/DHN oligomerization could be explored as a part of their molecular mechanism”