Abstract
Dweet mottle disease was first detected in Cleopatra mandarin CRC-270 in California and causes chlorotic blotching in Dweet tangor seedlings. Nagami kumquat SRA-153 induces similar symptoms in this indicator, but additionally causes vein clearing in several citrus species, stem pitting in Etrog citron, and bud-union crease when propagated on Troyer citrange. Citrus leaf blotch virus (CLBV) was found in this kumquat and its genomic RNA sequenced, thus allowing its detection by molecular hybridization and RT-PCR assays. Two Californian sources of Dweet mottle (DMV-931 and DMV-932) and kumquat SRA-153 were graft-inoculated on Pineapple sweet orange, Dweet tangor, Etrog citron and Nules Clementine and infected Clementine buds were then propagated on Carrizo citrange rootstock. Kumquat SRA-153 induced bud-union crease of Nules Clementine on citrange, vein clearing in Pineapple sweet orange, chlorotic blotching in Dweet tangor and stem pitting in Etrog citron, whereas Dweet mottle sources only induced blotching in Dweet tangor and stem pitting in Etrog citron. Fragments of the CLBV genome were RT-PCR amplified from indicator plants inoculated with either kumquat SRA-153 or Dweet mottle sources. The nucleotide identity between CLBV isolate SRA-153 and the DMV-931 and DMV-932 sources was 96.6% and 96.8% in a region of the RNA replicase gene, and 98.7% and 98.5% in the coat protein gene, respectively. These data and previous findings that Dweet mottle and CLBV are difficult to eliminate by shoot-tip grafting or thermotherapy, suggest that Dweet mottle may be caused by CLBV, and that, besides CLBV, a different pathogen causing bud-union crease and vein clearing may be present in kumquat SRA-153 but not in DMV-931 and DMV-932 sources.
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Claims 14
Claim · model-written summary
Kumquat SRA-153 caused a bud-union crease in Nules Clementine on citrange.
- Taxon
- Nules Clementinee0
- Material
- Nules Clementinee0
- Treatment
- Kumquat SRA-153e0
- Interactor
- Kumquat SRA-153e0pathogen
- Outcome
- bud-union creasee0
Not reportedComparatorDirection
Verbatim
e0“Kumquat SRA-153 induced bud-union crease of Nules Clementine on citrange”
Claim · model-written summary
Kumquat SRA-153 caused vein clearing in Pineapple sweet orange.
Not reportedComparatorDirection
Verbatim
e0“Kumquat SRA-153 induced bud-union crease of Nules Clementine on citrange, vein clearing in Pineapple sweet orange”
Claim · model-written summary
Kumquat SRA-153 caused chlorotic blotching in Dweet tangor.
Not reportedComparatorDirection
Verbatim
e0“Kumquat SRA-153 induced bud-union crease of Nules Clementine on citrange, vein clearing in Pineapple sweet orange, chlorotic blotching in Dweet tangor”
Claim · model-written summary
Kumquat SRA-153 caused stem pitting in Etrog citron.
Not reportedComparatorDirection
Verbatim
e0“Kumquat SRA-153 induced bud-union crease of Nules Clementine on citrange, vein clearing in Pineapple sweet orange, chlorotic blotching in Dweet tangor and stem pitting in Etrog citron”
Claim · model-written summary
Dweet mottle sources caused blotching in Dweet tangor.
Not reportedComparatorDirection
Verbatim
e0“Dweet mottle sources only induced blotching in Dweet tangor and stem pitting in Etrog citron.”
Claim · model-written summary
Dweet mottle sources caused stem pitting in Etrog citron.
Not reportedComparatorDirection
Verbatim
e0“Dweet mottle sources only induced blotching in Dweet tangor and stem pitting in Etrog citron.”
Claim · model-written summary
CLBV genome fragments were detected in plants inoculated with kumquat SRA-153.
- Taxon
- indicator plantse0
- Treatment
- kumquat SRA-153e0
- Interactor
- CLBVe0pathogen
- Outcome
- Fragments of the CLBV genome were RT-PCR amplifiede0
Not reportedDirection
Verbatim
e0“Fragments of the CLBV genome were RT-PCR amplified from indicator plants inoculated with either kumquat SRA-153 or Dweet mottle sources.”
Claim · model-written summary
CLBV genome fragments were detected in plants inoculated with Dweet mottle sources.
- Taxon
- indicator plantse0
- Treatment
- Dweet mottle sourcese0
- Interactor
- CLBVe0pathogen
- Outcome
- Fragments of the CLBV genome were RT-PCR amplifiede0
Not reportedDirection
Verbatim
e0“Fragments of the CLBV genome were RT-PCR amplified from indicator plants inoculated with either kumquat SRA-153 or Dweet mottle sources.”
Claim · model-written summary
SRA-153 and DMV-931 shared 96.6% nucleotide identity in the RNA replicase gene region.
- Material
- DMV-931e0
- Interactor
- CLBVe0pathogen
- Gene
- RNA replicase genee0
- Outcome
- nucleotide identitye0
- Value
- 96.6%e0
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nucleotide identity between CLBV isolate SRA-153 and the DMV-931 and DMV-932 sources was 96.6% and 96.8% in a region of the RNA replicase gene”
t0“Citrus Leaf Blotch Virus”
Claim · model-written summary
SRA-153 and DMV-932 shared 96.8% nucleotide identity in the RNA replicase gene region.
- Material
- DMV-932e0
- Interactor
- CLBVe0pathogen
- Gene
- RNA replicase genee0
- Outcome
- nucleotide identitye0
- Value
- 96.8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nucleotide identity between CLBV isolate SRA-153 and the DMV-931 and DMV-932 sources was 96.6% and 96.8% in a region of the RNA replicase gene”
t0“Citrus Leaf Blotch Virus”
Claim · model-written summary
SRA-153 and DMV-931 shared 98.7% nucleotide identity in the coat protein gene.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“98.7% and 98.5% in the coat protein gene, respectively.”
t0“Citrus Leaf Blotch Virus”
Claim · model-written summary
SRA-153 and DMV-932 shared 98.5% nucleotide identity in the coat protein gene.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“98.7% and 98.5% in the coat protein gene, respectively.”
t0“Citrus Leaf Blotch Virus”
Claim · model-written summary
The authors infer that CLBV may cause Dweet mottle disease.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggest that Dweet mottle may be caused by CLBV”
t0“Citrus Leaf Blotch Virus”
Claim · model-written summary
The authors suggest an additional pathogen may explain bud-union crease and vein clearing in kumquat SRA-153.
Verbatim
e0“a different pathogen causing bud-union crease and vein clearing may be present in kumquat SRA-153 but not in DMV-931 and DMV-932 sources.”