Abstract
Nitrogen (N) is one of the most important nutrients for plants and nitric oxide (NO) as a signaling plant growth regulator involved in nitrogen assimilation. Understanding the influence of exogenous NO on nitrogen metabolism at the gene expression and enzyme activity levels under different sources of nitrogen is vitally important for increasing nitrogen use efficiency (NUE). This study investigated the expression of key genes and enzymes in relation to nitrogen assimilation in two Australian wheat cultivars, a popular high NUE cv. Spitfire and a normal NUE cv. Westonia, under different combinations of nitrogen and sodium nitroprusside (SNP) as the NO donor. Application of NO increased the gene expressions and activities of nitrogen assimilation pathway enzymes in both cultivars at low levels of nitrogen. At high nitrogen supplies, the expressions and activities of N assimilation genes increased in response to exogenous NO only in cv. Spitfire but not in cv. Westonia. Exogenous NO caused an increase in leaf NO content at low N supplies in both cultivars, while under high nitrogen treatments, cv. Spitfire showed an increase under ammonium nitrate (NH4NO3) treatment but cv. Westonia was not affected. N assimilation gene expression and enzyme activity showed a clear relationship between exogenous NO, N concentration and N forms in primary plant nitrogen assimilation. Results reveal the possible role of NO and different nitrogen sources on nitrogen assimilation in Triticum aestivum plants.
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Claims 7
Claim · model-written summary
Exogenous NO increased gene expression and enzyme activities of the nitrogen assimilation pathway in both cultivars at low N.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- Application of NOe0
- Outcome
- gene expressions and activities of nitrogen assimilation pathway enzymese0
- Direction
- increasede0
- Qualifier
- at low levels of nitrogene0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels”
e0“Application of NO increased the gene expressions and activities of nitrogen assimilation pathway enzymes in both cultivars at low levels of nitrogen”
Claim · model-written summary
At high N, exogenous NO increased expression and activity of N assimilation genes in cv. Spitfire.
- Taxon
- wheatTriticum aestivumt0
- Material
- cv. Spitfiree1
- Treatment
- exogenous NOe1
- Outcome
- expressions and activities of N assimilation genese1
- Direction
- increasede1
- Qualifier
- At high nitrogen suppliese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels”
e1“At high nitrogen supplies, the expressions and activities of N assimilation genes increased in response to exogenous NO only in cv. Spitfire but not in cv. Westonia”
Claim · model-written summary
At high N, exogenous NO did not change expression and activity of N assimilation genes in cv. Westonia.
- Taxon
- wheatTriticum aestivumt0
- Material
- cv. Westoniae1
- Treatment
- exogenous NOe1
- Outcome
- expressions and activities of N assimilation genese1
- Direction
- no_changeinferrede1
- Qualifier
- At high nitrogen suppliese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels”
e1“At high nitrogen supplies, the expressions and activities of N assimilation genes increased in response to exogenous NO only in cv. Spitfire but not in cv. Westonia”
Claim · model-written summary
Exogenous NO caused an increase in leaf NO content at low N in both cultivars.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- leafe2
- Treatment
- Exogenous NOe2
- Outcome
- leaf NO contente2
- Direction
- increasee2
- Qualifier
- at low N suppliese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels”
e2“Exogenous NO caused an increase in leaf NO content at low N supplies in both cultivars”
Claim · model-written summary
Under high N, leaf NO content of cv. Spitfire increased under ammonium nitrate treatment.
- Taxon
- wheatTriticum aestivumt0
- Material
- cv. Spitfiree3
- Treatment
- ammonium nitrate (NH4NO3) treatmente3
- Outcome
- leaf NO contente2
- Direction
- increasee3
- Qualifier
- under high nitrogen treatmentse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels”
e2“Exogenous NO caused an increase in leaf NO content at low N supplies in both cultivars”
e3“under high nitrogen treatments, cv. Spitfire showed an increase under ammonium nitrate (NH4NO3) treatment but cv. Westonia was not affected”
Claim · model-written summary
Under high N, leaf NO content of cv. Westonia was not affected.
- Taxon
- wheatTriticum aestivumt0
- Material
- cv. Westoniae3
- Treatment
- Exogenous NOe2
- Outcome
- leaf NO contente2
- Direction
- no_changeinferrede3
- Qualifier
- under high nitrogen treatmentse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels”
e2“Exogenous NO caused an increase in leaf NO content at low N supplies in both cultivars”
e3“under high nitrogen treatments, cv. Spitfire showed an increase under ammonium nitrate (NH4NO3) treatment but cv. Westonia was not affected”
Claim · model-written summary
N assimilation gene expression and enzyme activity showed a clear relationship with exogenous NO, N concentration and N forms.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- exogenous NOe4
- Outcome
- N assimilation gene expression and enzyme activitye4
- Qualifier
- N concentration and N formse4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“How exogenous nitric oxide regulates nitrogen assimilation in wheat seedlings under different nitrogen sources and levels”
e4“N assimilation gene expression and enzyme activity showed a clear relationship between exogenous NO, N concentration and N forms in primary plant nitrogen assimilation”
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.