Abstract
To investigate the reasons for terminal bud death, the relationship between terminal bud death and programmed cell death in Paulownia was studied using morphocytology and DNA ladder techniques with one-year-old seedlings of Paulownia spp. The results showed that there were no characteristic morphological changes of the chromatin/chromosomes or nucleus, such as chromatin condensation, nuclear shrinkage, distortion, or degradation after DAPI (4',6-diamidino-2-phenylindo-le) staining, and DNA laddering was not found in cells after agarose gel electrophoresis. Preliminarily results suggest that the terminal bud death of Paulownia spp. is not clearly related to programmed cell death.
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Claims 3
Claim · model-written summary
Characteristic nuclear and chromatin changes were absent.
- Outcome
- no characteristic morphological changes of the chromatin/chromosomes or nucleuse0
Verbatim
t0“Paulownia spp.”
e0“there were no characteristic morphological changes of the chromatin/chromosomes or nucleus”
Claim · model-written summary
DNA laddering was absent.
- Outcome
- DNA laddering was not founde0
Verbatim
t0“Paulownia spp.”
e0“DNA laddering was not found in cells after agarose gel electrophoresis”
Claim · model-written summary
Terminal bud death was not clearly related to programmed cell death.
Not reportedDirection
Verbatim
e0“the terminal bud death of Paulownia spp. is not clearly related to programmed cell death”