Abstract
Transgenic plums, Prunus domestica L. clone C5, were inoculated by bud grafting with Plum pox virus (PPV-Rec, recombinant strain originated from plum), PPV-Rec + Apple chlorotic leafspot virus (ACLSV), PPV-Rec + Prune dwarf virus (PDV), and PPV-Rec + ACLSV + PDV. Non-inoculated transgenic plums served as controls. Plants were grown in an open field for 5 years. They were evaluated by visible symptoms, by DAS-ELISA and RT-PCR. Mild PPV symptoms, diffuse spots or rings appeared two years after inoculation in some leaves of plants artificially inoculated with PPV-Rec, PPV-Rec + ACLSV, PPV-Rec + PDV, and PPV-Rec + ACLSV + PDV. Severe PPV symptoms appeared in leaves of shoots growing from infected buds used for inoculation. During the following three years, further weakening of PPV symptoms was observed in transgenic plants. In 2007, very mild PPV symptoms were found in only a few leaves, and over 60%, resp. 70% of the C5 trees showed no PPV symptoms. The presence of PPV was confirmed by ELISA, ISEM and RT-PCR. No difference in PPV symptoms was observed between PPV-Rec and combinations PPV-Rec + ACLSV, PPV-Rec + PDV, PPV-Rec + ACLSV + PDV. No symptoms of ACLSV appeared in combinations of ACLSV with PPV-Rec and PPV-Rec + PDV during 2004-2007, but the presence of ACLSV in leaves of transgenic plants clone C5 was proved by ELISA and RT-PCR. Neither synergistic nor antagonistic effects of ACLSV on PPV-Rec were observed. No symptoms of PDV appeared in combinations of viruses with PDV during 2004-2007. PDV was not detected by ELISA, and the presence of PDV was uncertain by RT-PCR in most of inoculated trees in 2006 and 2007. The results of RT-PCR will be further confirmed by sequence analysis and discussed. These results suggest a possible antagonistic interaction between PPV-Rec and PDV in plum clone C5.
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Claims 12
Claim · model-written summary
Mild PPV symptoms (diffuse spots or rings) appeared in some leaves two years after inoculation in transgenic plum.
- Taxon
- Prunus domestica L.
Prunus domesticae0 - Material
- clone C5e0
- Interactor
- PPVe1pathogen
- Outcome
- Mild PPV symptoms, diffuse spots or ringse1
- Qualifier
- two years after inoculation in some leavese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plums, Prunus domestica L. clone C5”
e1“Mild PPV symptoms, diffuse spots or rings appeared two years after inoculation in some leaves”
Claim · model-written summary
Severe PPV symptoms appeared in leaves of shoots growing from infected buds used for inoculation.
- Taxon
- Prunus domestica L.Prunus domesticae0
- Material
- clone C5e0
- Interactor
- PPVe1pathogen
- Outcome
- Severe PPV symptomse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plums, Prunus domestica L. clone C5”
e1“Severe PPV symptoms appeared in leaves of shoots growing from infected buds used for inoculation”
Claim · model-written summary
PPV symptoms weakened further in the transgenic plants during the following three years.
- Taxon
- Prunus domestica L.Prunus domesticae0
- Material
- clone C5e0
- Interactor
- PPVe1pathogen
- Outcome
- weakening of PPV symptomse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plums, Prunus domestica L. clone C5”
e1“further weakening of PPV symptoms was observed in transgenic plants”
Claim · model-written summary
In 2007 over 60% and 70% (respectively) of C5 trees showed no PPV symptoms.
- Taxon
- Prunus domestica L.Prunus domesticae0
- Material
- clone C5e0
- Interactor
- PPVe1pathogen
- Outcome
- no PPV symptomse1
- Value
- over 60%, resp. 70%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plums, Prunus domestica L. clone C5”
e1“over 60%, resp. 70% of the C5 trees showed no PPV symptoms”
Claim · model-written summary
The presence of PPV was confirmed by ELISA, ISEM and RT-PCR.
- Taxon
- Prunus domestica L.Prunus domesticae0
- Material
- clone C5e0
- Interactor
- PPVe1pathogen
- Outcome
- presence of PPVe1
- Qualifier
- ELISA, ISEM and RT-PCRe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plums, Prunus domestica L. clone C5”
e1“The presence of PPV was confirmed by ELISA, ISEM and RT-PCR”
Claim · model-written summary
PPV symptoms did not differ between PPV-Rec alone and the combinations PPV-Rec + ACLSV, PPV-Rec + PDV and PPV-Rec + ACLSV + PDV.
- Taxon
- Prunus domestica L.Prunus domesticae0
- Material
- clone C5e0
- Treatment
- combinations PPV-Rec + ACLSV, PPV-Rec + PDV, PPV-Rec + ACLSV + PDVe1
- Interactor
- PPV-Rece1pathogen
- Second interactor
- ACLSV
Trichovirus malie1pathogen - Outcome
- PPV symptomse1
- Direction
- No differencee1
- Qualifier
- PPV-Rec + PDV, PPV-Rec + ACLSV + PDVe1
- Comparator
- PPV-Rece1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plums, Prunus domestica L. clone C5”
e1“No difference in PPV symptoms was observed between PPV-Rec and combinations PPV-Rec + ACLSV, PPV-Rec + PDV, PPV-Rec + ACLSV + PDV”
Claim · model-written summary
No ACLSV symptoms appeared in combinations of ACLSV with PPV-Rec and with PPV-Rec + PDV during 2004-2007.
- Taxon
- Prunus domestica L.Prunus domesticae0
- Material
- clone C5e0
- Interactor
- ACLSVTrichovirus malie1pathogen
- Second interactor
- PPV-Rece1pathogen
- Outcome
- No symptoms of ACLSVe1
- Qualifier
- during 2004-2007e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plums, Prunus domestica L. clone C5”
e1“No symptoms of ACLSV appeared in combinations of ACLSV with PPV-Rec and PPV-Rec + PDV during 2004-2007”
Claim · model-written summary
ACLSV was present in leaves of transgenic plum clone C5, as proved by ELISA and RT-PCR.
- Taxon
- Prunus domestica L.Prunus domesticae0
- Material
- clone C5e0
- Organ / stage
- leavese1
- Interactor
- ACLSVTrichovirus malie1pathogen
- Outcome
- presence of ACLSVe1
- Qualifier
- ELISA and RT-PCRe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plums, Prunus domestica L. clone C5”
e1“the presence of ACLSV in leaves of transgenic plants clone C5 was proved by ELISA and RT-PCR”
Claim · model-written summary
No synergistic or antagonistic effects of ACLSV on PPV-Rec were observed.
- Taxon
- Prunus domestica L.Prunus domesticae0
- Material
- clone C5e0
- Treatment
- ACLSVe1
- Interactor
- PPV-Rece1pathogen
- Second interactor
- ACLSVTrichovirus malie1pathogen
- Outcome
- synergistic nor antagonistic effects of ACLSV on PPV-Rece1
- Direction
- no_changeinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plums, Prunus domestica L. clone C5”
e1“Neither synergistic nor antagonistic effects of ACLSV on PPV-Rec were observed”
Claim · model-written summary
No PDV symptoms appeared in combinations of viruses with PDV during 2004-2007.
- Taxon
- Prunus domestica L.Prunus domesticae0
- Material
- clone C5e0
- Interactor
- PDVe1pathogen
- Outcome
- No symptoms of PDVe1
- Qualifier
- during 2004-2007e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plums, Prunus domestica L. clone C5”
e1“No symptoms of PDV appeared in combinations of viruses with PDV during 2004-2007”
Claim · model-written summary
PDV was not detected by ELISA and its presence was uncertain by RT-PCR in most inoculated trees in 2006 and 2007.
- Taxon
- Prunus domestica L.Prunus domesticae0
- Material
- clone C5e0
- Interactor
- PDVe1pathogen
- Outcome
- PDV was not detected by ELISA, and the presence of PDV was uncertain by RT-PCRe1
- Qualifier
- in 2006 and 2007e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plums, Prunus domestica L. clone C5”
e1“PDV was not detected by ELISA, and the presence of PDV was uncertain by RT-PCR in most of inoculated trees in 2006 and 2007”
Claim · model-written summary
The results suggest a possible antagonistic interaction between PPV-Rec and PDV in plum clone C5.
- Taxon
- plumPrunus domesticae1
- Material
- clone C5e1
- Interactor
- PPV-Rece1pathogen
- Second interactor
- PDVe1pathogen
- Outcome
- possible antagonistic interaction between PPV-Rec and PDVe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These results suggest a possible antagonistic interaction between PPV-Rec and PDV in plum clone C5”
The same questions in other papers
pruning · disease · Prunus domestica (plum)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.