Abstract
The Oomycete Plasmopara viticola is responsible for downy mildew, which is one of the most damaging grapevine diseases. Due to the strictly biotrophic way of life of P. viticola, its metabolome is relatively poorly characterized. In this work, we have used a mass spectrometry-based non-targeted metabolomic approach to identify potential Plasmopara-specific metabolites. This has led to the characterization and structural elucidation of compounds belonging to three families of atypical lipids, which are not detected in healthy grapevine tissues. These lipids include ceramides and derivatives of arachidonic and eicosapentaenoic acid, most of which had not been previously described in Oomycetes. Furthermore, we show that these lipids can be detected in Plasmopara-infected tissues at very early stages of the infection process, long before the appearance the first visible symptoms of the disease. Therefore, the potential use of these specific lipids as markers to monitor the development of P. viticola is discussed.
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Claims 4
Claim · model-written summary
Three families of atypical lipids were characterized and absent from healthy grapevine tissue.
- Outcome
- three families of atypical lipidse0
- Value
- three familiese0
- Comparator
- healthy grapevine tissuese0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“compounds belonging to three families of atypical lipids, which are not detected in healthy grapevine tissues”
t0“Grapevine”
Claim · model-written summary
The identified lipids include ceramides and derivatives of arachidonic and eicosapentaenoic acid.
- Outcome
- ceramides and derivatives of arachidonic and eicosapentaenoic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These lipids include ceramides and derivatives of arachidonic and eicosapentaenoic acid”
t0“Grapevine”
Claim · model-written summary
Most identified lipids had not previously been described in Oomycetes.
- Outcome
- not been previously described in Oomycetese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most of which had not been previously described in Oomycetes”
t0“Grapevine”
Claim · model-written summary
These lipids were detected in Plasmopara-infected tissue before visible disease symptoms.
- Interactor
- Plasmopara
Plasmoparae0pathogen - Outcome
- these lipids can be detected in Plasmopara-infected tissuese0
- Comparator
- long before the appearance the first visible symptoms of the diseasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these lipids can be detected in Plasmopara-infected tissues at very early stages of the infection process, long before the appearance the first visible symptoms of the disease”
t0“Grapevine”