Abstract
Background and Objective: Regeneration of plant through tissue culture technique is a critical process in transformation of plants. This study sought to establish the effect of genotype and auxin concentration on callus induction from different sweet sorghum genotypes (IESV92008DL, IESV92001DL, IESV92021DL, ICSV700 and ICSV93048). Materials and Methods: In this study, MS medium supplemented with five levels of the hormone 2,4-D (0, 1, 2, 4 and 6 mg LG 1 ) to assess the effect of the hormone 2,4-D on callus induction and regeneration was used. Results: The highest callus induction frequency was observed at 2 mg LG 1 2,4-D for all the genotypes, IESV92008 gave the highest callus induction frequency among all the genotypes at 2 mg LG 1 2,4-D. The lowest callus induction frequency was observed 0 and 6 mg LG 1 for all the genotypes, the two genotypes ICSV700 and IESV92021 were observed to give the lowest callus induction frequency among all the genotypes used in this study. Embryogenic callus induction frequency was observed to be higher at 2 mg LG 1 2,4-D and the genotype IESV92008 gave the highest callus induction frequency among all the genotypes used. Induction of shoots was achieved in MS medium supplemented with 3 mg LG 1 6BA and 1 mg LG 1 IAA, the highest regeneration efficiency was obtained from the three genotypes ICSV93048, IESV92008 and IESV92001, respectively. Conclusion: This study discovers the importance of the auxin 2,4-D on callus induction and regeneration of sweet sorghum and this will help the researcher to develop protocols for transformation of sweet sorghum.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
At 2 mg LG 1 2,4-D, callus induction frequency was highest across genotypes.
Not reportedComparator
Verbatim
t0“Sweet Sorghum”
e0“The highest callus induction frequency was observed at 2 mg LG 1 2,4-D for all the genotypes”
Claim · model-written summary
IESV92008 had the highest callus induction frequency among genotypes at 2 mg LG 1 2,4-D.
Verbatim
t0“Sweet Sorghum”
e0“IESV92008 gave the highest callus induction frequency among all the genotypes at 2 mg LG 1 2,4-D”
Claim · model-written summary
At 0 mg LG 1 2,4-D, callus induction was lowest across genotypes.
Not reportedComparator
Verbatim
t0“Sweet Sorghum”
e0“The lowest callus induction frequency was observed 0 and 6 mg LG 1 for all the genotypes”
Claim · model-written summary
At 6 mg LG 1 2,4-D, callus induction was lowest across genotypes.
Not reportedComparator
Verbatim
t0“Sweet Sorghum”
e0“The lowest callus induction frequency was observed 0 and 6 mg LG 1 for all the genotypes”
Claim · model-written summary
ICSV700 had among the lowest callus induction frequencies.
Verbatim
t0“Sweet Sorghum”
e0“the two genotypes ICSV700 and IESV92021 were observed to give the lowest callus induction frequency among all the genotypes used in this study”
Claim · model-written summary
IESV92021 had among the lowest callus induction frequencies.
Verbatim
t0“Sweet Sorghum”
e0“the two genotypes ICSV700 and IESV92021 were observed to give the lowest callus induction frequency among all the genotypes used in this study”
Claim · model-written summary
Embryogenic callus induction frequency was higher at 2 mg LG 1 2,4-D.
- Taxon
- Sweet Sorghumt0
- Treatment
- 2 mg LG 1 2,4-De0
- Outcome
- Embryogenic callus induction frequencye0
- Direction
- highere0
Not reportedComparator
Verbatim
t0“Sweet Sorghum”
e0“Embryogenic callus induction frequency was observed to be higher at 2 mg LG 1 2,4-D”
Claim · model-written summary
IESV92008 had the highest embryogenic callus induction frequency among genotypes.
Verbatim
t0“Sweet Sorghum”
e0“the genotype IESV92008 gave the highest callus induction frequency among all the genotypes used”
Claim · model-written summary
Shoots were induced on MS medium with 3 mg LG 1 6BA and 1 mg LG 1 IAA.
- Taxon
- Sweet Sorghumt0
- Treatment
- MS medium supplemented with 3 mg LG 1 6BA and 1 mg LG 1 IAAe0
- Outcome
- Induction of shoots was achievede0
Not reportedDirection
Verbatim
t0“Sweet Sorghum”
e0“Induction of shoots was achieved in MS medium supplemented with 3 mg LG 1 6BA and 1 mg LG 1 IAA”
Claim · model-written summary
ICSV93048 was among the three genotypes with highest regeneration efficiency.
Verbatim
t0“Sweet Sorghum”
e0“the highest regeneration efficiency was obtained from the three genotypes ICSV93048, IESV92008 and IESV92001”
Claim · model-written summary
IESV92008 was among the three genotypes with highest regeneration efficiency.
Verbatim
t0“Sweet Sorghum”
e0“the highest regeneration efficiency was obtained from the three genotypes ICSV93048, IESV92008 and IESV92001”
Claim · model-written summary
IESV92001 was among the three genotypes with highest regeneration efficiency.
Verbatim
t0“Sweet Sorghum”
e0“the highest regeneration efficiency was obtained from the three genotypes ICSV93048, IESV92008 and IESV92001”
The same questions in other papers
2,4-D · growth · SorghumPositive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.