Abstract
Leaf explants of critically endangered medicinal plant Swertia chirayita were cultured in Murashige and Skoog medium fortified with various concentrations of 2,4-Diclorophenoxyacetic acid (2,4-D), 6-Benzylaminopurine (BAP) and Gibberalic acid (GA3). The best callus induction was obtained in 2,4-D (15.0 µM) after 60 days of culture period. After 30 days of first subculture, maximum 32 microshoots were formed in a lump of callus in combination of BAP (10.0 µM) with GA3 (5.0 µM). On the other hand maximum 25 microshoots were formed from a lump of callus in BAP (5.0 µM) used alone. The multiple shoots developed in combination of BAP with GA3 were found poor/thin in comparison to BAP alone. Therefore, the further subculture was conducted in BAP (5.0 µM) and approximately 210 microshoots per lump of callus were obtained within 3–4 months of culture. Root induction was observed in all microshoots cultured in various concentrations of auxins (Indole-3-butyric acid, IBA and Indole-3-acetic acid, IAA) after 45 days of culture, however, the higher mean number of roots (50) per microshoot were formed in IBA (5.0 µM). After a month, well rooted plantlets were successfully transplanted and established (58.70%) in mixture of soil, sand and FYM (2:1:1) under partially shade (75% agro shade net) condition in nursery at Pothibasa (2200 m a.s.l.).
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Claims 8
Claim · model-written summary
2,4-D at 15.0 µM gave the best callus induction after 60 days.
- Taxon
- Swertia chirayita
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Swertia chirayitat0 - Treatment
- 2,4-D (15.0 µM)e0
- Outcome
- callus inductione0
- Direction
- beste0
- Qualifier
- after 60 days of culture periode0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The best callus induction was obtained in 2,4-D (15.0 µM) after 60 days of culture period.”
t0“Swertia chirayita”
Claim · model-written summary
BAP plus GA3 produced a maximum of 32 microshoots per callus lump.
- Taxon
- Swertia chirayitaSwertia chirayitat0
- Treatment
- combination of BAP (10.0 µM) with GA3 (5.0 µM)e0
- Outcome
- microshootse0
- Direction
- maximume0
- Value
- 32 microshootse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum 32 microshoots were formed in a lump of callus in combination of BAP (10.0 µM) with GA3 (5.0 µM)”
t0“Swertia chirayita”
Claim · model-written summary
BAP alone yielded a maximum of 25 microshoots per callus lump.
- Taxon
- Swertia chirayitaSwertia chirayitat0
- Treatment
- BAP (5.0 µM)e0
- Outcome
- microshootse0
- Direction
- maximume0
- Value
- 25 microshootse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum 25 microshoots were formed from a lump of callus in BAP (5.0 µM) used alone.”
t0“Swertia chirayita”
Claim · model-written summary
Shoots produced with BAP and GA3 were thin compared with those from BAP alone.
- Taxon
- Swertia chirayitaSwertia chirayitat0
- Treatment
- combination of BAP with GA3e0
- Outcome
- multiple shootse0
- Direction
- poor/thine0
- Comparator
- BAP alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The multiple shoots developed in combination of BAP with GA3 were found poor/thin in comparison to BAP alone.”
t0“Swertia chirayita”
Claim · model-written summary
Further culture with BAP at 5.0 µM produced approximately 210 microshoots per callus lump within 3–4 months.
- Taxon
- Swertia chirayitaSwertia chirayitat0
- Treatment
- BAP (5.0 µM)e0
- Outcome
- microshootse0
- Value
- approximately 210 microshoots per lump of calluse0
- Qualifier
- within 3–4 months of culturee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“further subculture was conducted in BAP (5.0 µM) and approximately 210 microshoots per lump of callus were obtained within 3–4 months of culture.”
t0“Swertia chirayita”
Claim · model-written summary
All microshoots cultured with auxins formed roots after 45 days.
- Taxon
- Swertia chirayitaSwertia chirayitat0
- Treatment
- various concentrations of auxins (Indole-3-butyric acid, IBA and Indole-3-acetic acid, IAA)e0
- Outcome
- Root inductione0
- Qualifier
- after 45 days of culturee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root induction was observed in all microshoots cultured in various concentrations of auxins (Indole-3-butyric acid, IBA and Indole-3-acetic acid, IAA) after 45 days of culture”
t0“Swertia chirayita”
Claim · model-written summary
IBA at 5.0 µM gave the higher mean number of roots, 50 per microshoot.
- Taxon
- Swertia chirayitaSwertia chirayitat0
- Treatment
- IBA (5.0 µM)e0
- Outcome
- mean number of rootse0
- Direction
- highere0
- Value
- 50e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the higher mean number of roots (50) per microshoot were formed in IBA (5.0 µM)”
t0“Swertia chirayita”
Claim · model-written summary
Rooted plantlets established at 58.70% after transplantation into a soil, sand, and FYM mix under nursery shade.
- Taxon
- Swertia chirayitaSwertia chirayitat0
- Treatment
- mixture of soil, sand and FYM (2:1:1)e0
- Outcome
- establishede0
- Value
- 58.70%e0
- Setting
- nursery at Pothibasae0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“well rooted plantlets were successfully transplanted and established (58.70%) in mixture of soil, sand and FYM (2:1:1) under partially shade (75% agro shade net) condition in nursery at Pothibasa (2200 m a.s.l.).”
t0“Swertia chirayita”
The same questions in other papers
2,4-D · growth · Swertia chirayitaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.