Abstract
BACKGROUND: Cryopreservation of germplasm in liquid nitrogen is an ideal technique for the longer term storage of plant genetic material, including medicinal species. OBJECTIVE: To develop a somatic embryo production system for the medicinal species Swertia chirayita and to evaluate their potential for storage in liquid nitrogen (-196 degree C). MATERIALS AND METHODS: An efficient protocol of somatic embryogenesis was developed for the first time using leaves of in-vitro grown shoots of S. chirayita. Somatic embryos were then encapsulated in 3% sodium alginate, 0.85 M sucrose and 100 mM calcium chloride for synthetic seed production and subjected to cryopreservation. Marker medicinal compounds were determined by RP-HPLC analysis. RESULTS: A medium containing 1 mg/L 2,4-D+ 0.5 mg/L BAP+ 0.5 mg/L TDZ was found to stimulate the highest callus induction. Somatic embryos were recovered after 5 weeks, when cultured on the same media. Synthetic seeds were dehydrated and immersed in liquid nitrogen for 1 h. Cryopreserved synthetic seeds were successfully revived and germinated on MS media supplemented with 1 mg/L IBA+ 2 mg/L KN + 3 mg/L GA3 in which 93.3% somatic embryos differentiated into shoots. One month old in-vitro grown shoots from cryopreserved somatic embryos had similar marker medicinal compounds, such as amarogentin (4.72 ± 0.11 ug/mg) and mangiferin (14.54 ± 0.05 ug/mg), as control material. CONCLUSION: This protocol offers vast scope for multiplying material of an endangered medicinal herb and subsequent cryopreservation. doi.org/10.54680/fr22510110612.
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Claims 6
Claim · model-written summary
A medium with 2,4-D, BAP and TDZ at the stated rates gave the highest callus induction.
- Taxon
- Swertia Chirayita
.jpg?width=60)
Swertia chirayitat0 - Treatment
- 1 mg/L 2,4-D+ 0.5 mg/L BAP+ 0.5 mg/L TDZe0
- Outcome
- callus inductione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“A medium containing 1 mg/L 2,4-D+ 0.5 mg/L BAP+ 0.5 mg/L TDZ was found to stimulate the highest callus induction”
t0“Swertia Chirayita”
Claim · model-written summary
Somatic embryos were recovered after 5 weeks on the same medium.
- Taxon
- Swertia ChirayitaSwertia chirayitat0
- Outcome
- Somatic embryos were recoverede0
- Value
- 5 weekse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryos were recovered after 5 weeks, when cultured on the same media”
t0“Swertia Chirayita”
Claim · model-written summary
Cryopreserved synthetic seeds revived and germinated, with 93.3% of somatic embryos differentiating into shoots.
- Taxon
- Swertia ChirayitaSwertia chirayitat0
- Treatment
- 1 mg/L IBA+ 2 mg/L KN + 3 mg/L GA3e0
- Outcome
- somatic embryos differentiated into shootse0
- Value
- 93.3%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cryopreserved synthetic seeds were successfully revived and germinated on MS media supplemented with 1 mg/L IBA+ 2 mg/L KN + 3 mg/L GA3 in which 93.3% somatic embryos differentiated into shoots”
t0“Swertia Chirayita”
Claim · model-written summary
Cryopreserved embryo-derived shoots had similar amarogentin content to controls, at 4.72 ± 0.11 ug/mg.
- Taxon
- Swertia ChirayitaSwertia chirayitat0
- Organ / stage
- One month old in-vitro grown shootse0
- Outcome
- amarogentine0
- Direction
- similare0
- Value
- 4.72 ± 0.11 ug/mge0
- Comparator
- control materiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One month old in-vitro grown shoots from cryopreserved somatic embryos had similar marker medicinal compounds, such as amarogentin (4.72 ± 0.11 ug/mg) and mangiferin (14.54 ± 0.05 ug/mg), as control material”
t0“Swertia Chirayita”
Claim · model-written summary
Cryopreserved embryo-derived shoots had similar mangiferin content to controls, at 14.54 ± 0.05 ug/mg.
- Taxon
- Swertia ChirayitaSwertia chirayitat0
- Organ / stage
- One month old in-vitro grown shootse0
- Outcome
- mangiferine0
- Direction
- similare0
- Value
- 14.54 ± 0.05 ug/mge0
- Comparator
- control materiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One month old in-vitro grown shoots from cryopreserved somatic embryos had similar marker medicinal compounds, such as amarogentin (4.72 ± 0.11 ug/mg) and mangiferin (14.54 ± 0.05 ug/mg), as control material”
t0“Swertia Chirayita”
Claim · model-written summary
The protocol was proposed for multiplying and cryopreserving this endangered herb.
- Taxon
- Swertia ChirayitaSwertia chirayitat0
- Outcome
- multiplying material of an endangered medicinal herb and subsequent cryopreservatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This protocol offers vast scope for multiplying material of an endangered medicinal herb and subsequent cryopreservation”
t0“Swertia Chirayita”
The same questions in other papers
2,4-D · growth · Swertia chirayitaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.