Abstract
Summary Mechanical damage affects tea plant growth and the quality of tea‐leaf products. However, data on the wound response of tea leaves at the transcriptome level remain scarce. In this study, the transcriptome of wound‐induced tea leaves was analysed by RNA ‐Seq and some of their secondary metabolites were determined using high‐performance liquid chromatography ( HPLC ) technology. The results showed that 129610 non‐redundant transcripts and 60361 unigenes were obtained, of which 100834 transcripts (77.80% of all transcripts) were annotated by sequence similarity searching against public databases and 28776 transcripts could be potentially new genes or novel transcripts from selective splicing. A total of 19214 differentially expressed transcripts ( DET s) were selected from the data set. DET functions involved various physiological and molecular biological processes in tea plant including various transcripts encoding protease inhibitors ( PI s), pathogenesis‐related proteins ( PR s) and enzymes related to catechin, caffeine and terpenoid metabolic pathways. Moreover, the contents of some polyphenols and caffeine were also validated in tea leaves after an increase in wounding. The transcriptome analysis revealed a reprogramming process in tea plants during wounding that involved various physiological and molecular biological processes, which can increase the resistance of plants against biotic and abiotic stress and affect the quality of tea‐leaf products. Our results not only provide systematic insight into the defence responses in tea plants during mechanical damage, but also are beneficial for the scientific cultivation of tea.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
The tea leaf transcriptome yielded 129610 non-redundant transcripts and 60361 unigenes.
Verbatim
e0“129610 non‐redundant transcripts and 60361 unigenes were obtained”
t0“tea plant”
Claim · model-written summary
Sequence similarity annotated 100834 tea transcripts, 77.80% of all transcripts.
Verbatim
e0“100834 transcripts (77.80% of all transcripts) were annotated by sequence similarity searching against public databases”
t0“tea plant”
Claim · model-written summary
A further 28776 tea transcripts could be new genes or alternative splice transcripts.
Verbatim
e0“28776 transcripts could be potentially new genes or novel transcripts from selective splicing”
t0“tea plant”
Claim · model-written summary
The tea transcriptome dataset contained 19214 differentially expressed transcripts.
Verbatim
e0“A total of 19214 differentially expressed transcripts ( DET s) were selected from the data set”
t0“tea plant”
Claim · model-written summary
Wound-responsive tea transcripts included protease inhibitors and pathogenesis-related proteins.
Verbatim
e0“various transcripts encoding protease inhibitors ( PI s), pathogenesis‐related proteins ( PR s) and enzymes related to catechin, caffeine and terpenoid metabolic pathways”
t0“tea plant during mechanical damage”
Claim · model-written summary
Wound-responsive tea transcripts included enzymes of catechin, caffeine, and terpenoid pathways.
Verbatim
e0“enzymes related to catechin, caffeine and terpenoid metabolic pathways”
t0“tea plant”
Claim · model-written summary
The contents of some tea leaf polyphenols and caffeine changed with increased wounding.
- Taxon
- tea plantt0
- Organ / stage
- tea leavese0
- Treatment
- increase in woundinge0
- Outcome
- contents of some polyphenols and caffeinee0
Not reportedDirection
Verbatim
e0“the contents of some polyphenols and caffeine were also validated in tea leaves after an increase in wounding”
t0“tea plant”
Claim · model-written summary
Transcriptome analysis indicated reprogramming of tea physiology and molecular processes during wounding.
Verbatim
e0“The transcriptome analysis revealed a reprogramming process in tea plants during wounding that involved various physiological and molecular biological processes”