Abstract
Hydration⁻dehydration cycles can frequently cause stress to seeds, but can also be used to improve germination. However, the molecular basis of the stress caused is poorly understood. Herein, we examine the effects of hydration⁻dehydration cycles on seed viability and profile the membrane glycerolipid molecular species. We find that seed viability was not affected during the first two cycles, but significantly decreased as further cycles were applied, until all viability was lost. The abundances of seven glycerolipid classes increased and decreased through hydration and dehydration, respectively, but the phosphatidic acid and diacylglycerol abundances changed in the opposite sense, while total glycerolipid contents remained constant. This suggests that during hydration⁻dehydration cycles, turnover of glycerolipid metabolite pools take place, while no significant lipid synthesis or degradation is involved. As further hydration⁻dehydration cycles occurred, lipid unsaturation increased, plastidic lipids decreased, and phosphatidylserine acyl chains lengthened. The latter two could be lethal for seeds. Our findings reveal a novel model of membrane lipid changes, and provide new insights into the responses of seeds to hydration⁻dehydration cycles.
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Claims 8
Claim · model-written summary
Seed viability was unaffected during the first two cycles.
Not reportedTaxonComparator
Verbatim
e0“seed viability was not affected during the first two cycles”
Claim · model-written summary
Further cycles significantly decreased viability until none remained.
Not reportedTaxonComparator
Verbatim
e0“significantly decreased as further cycles were applied, until all viability was lost.”
t0“Seed Viability”
Claim · model-written summary
Seven glycerolipid classes rose with hydration and fell with dehydration.
- Treatment
- hydration and dehydratione0
- Outcome
- abundances of seven glycerolipid classese0
- Direction
- increased and decreasede0
Not reportedTaxonComparator
Verbatim
e0“The abundances of seven glycerolipid classes increased and decreased through hydration and dehydration, respectively”
Claim · model-written summary
Phosphatidic acid and diacylglycerol abundances changed oppositely.
- Treatment
- hydration and dehydratione0
- Outcome
- phosphatidic acid and diacylglycerol abundancese0
- Direction
- opposite sensee0
Not reportedTaxonComparator
Verbatim
e0“The abundances of seven glycerolipid classes increased and decreased through hydration and dehydration, respectively, but the phosphatidic acid and diacylglycerol abundances changed in the opposite sense”
Claim · model-written summary
Total glycerolipid contents stayed constant.
- Treatment
- hydration and dehydratione0
- Outcome
- total glycerolipid contentse0
- Direction
- remained constante0
Not reportedTaxonComparator
Verbatim
e0“The abundances of seven glycerolipid classes increased and decreased through hydration and dehydration, respectively, but the phosphatidic acid and diacylglycerol abundances changed in the opposite sense, while total glycerolipid contents remained constant.”
Claim · model-written summary
Additional cycles increased lipid unsaturation.
Not reportedTaxonComparator
Verbatim
e0“As further hydration⁻dehydration cycles occurred, lipid unsaturation increased”
Claim · model-written summary
Additional cycles decreased plastidic lipids.
Not reportedTaxonComparator
Verbatim
e0“As further hydration⁻dehydration cycles occurred, lipid unsaturation increased, plastidic lipids decreased”
Claim · model-written summary
Additional cycles lengthened phosphatidylserine acyl chains.
- Treatment
- further hydration⁻dehydration cyclese0
- Outcome
- phosphatidylserine acyl chainse0
- Direction
- lengthenede0
Not reportedTaxonComparator
Verbatim
e0“As further hydration⁻dehydration cycles occurred, lipid unsaturation increased, plastidic lipids decreased, and phosphatidylserine acyl chains lengthened.”