Abstract
Heterotrimeric G proteins composed of α, β, and γ subunits are central signal transducers mediating the cellular response to multiple stimuli in eukaryotes. Plant Gγ subunits, divided into A, B, and C three structurally distinct types, provide proper cellular localization and functional specificity to the heterotrimer complex. Here, we demonstrate that a type C Gγ subunit CsGG3.2 is involved in the regulation of the CBF regulon and plant tolerance to cold stresses in cucumber (Cucumis sativus L.). We showed that CsGG3.2 transcript abundance was positively induced by cold treatments. Transgenic cucumber plants (T1) constitutively overexpressing CsGG3.2 exhibits tolerance to chilling conditions and increased expression of CBF genes and their regulon genes. Antioxidative enzymes, i.e., superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and glutathione reductase (GR) activities increased in cold-stressed transgenic plants. The reactive oxygen species (ROS), Oxygen free radical (OFR) and H2O2, production, as well as membrane lipid peroxidation (MDA) production decreased in transgenic plants, suggesting a better antioxidant system to cope the oxidative-damages caused by cold stress. These findings provide evidence for a critical role of CsGG3.2 in regulating of cold signal transduction in plant cells.
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Claims 7
Claim · model-written summary
Cold induced CsGG3.2 transcript abundance.
- Taxon
- cucumber
Cucumis sativust0 - Treatment
- cold treatmentse0
- Gene
- CsGG3.2e0
- Outcome
- CsGG3.2 transcript abundancee0
- Direction
- positively inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsGG3.2 transcript abundance was positively induced by cold treatments”
t0“Cucumber”
Claim · model-written summary
CsGG3.2 overexpression increased chilling tolerance.
- Taxon
- cucumberCucumis sativuse0
- Material
- Transgenic cucumber plants (T1)e0
- Treatment
- constitutively overexpressing CsGG3.2e0
- Gene
- CsGG3.2e0
- Outcome
- tolerance to chilling conditionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic cucumber plants (T1) constitutively overexpressing CsGG3.2 exhibits tolerance to chilling conditions”
Claim · model-written summary
CsGG3.2 overexpression increased CBF and regulon gene expression.
- Taxon
- cucumberCucumis sativust0
- Treatment
- constitutively overexpressing CsGG3.2e0
- Gene
- CBF genese0
- Outcome
- expression of CBF genes and their regulon genese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“constitutively overexpressing CsGG3.2 exhibits tolerance to chilling conditions and increased expression of CBF genes and their regulon genes”
t0“Cucumber”
Claim · model-written summary
Cold stressed transgenic plants had higher SOD, CAT, and POD activities.
- Taxon
- cucumberCucumis sativust0
- Treatment
- cold-stressed transgenic plantse0
- Outcome
- superoxide dismutase (SOD), catalase (CAT), peroxidase (POD)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and glutathione reductase (GR) activities increased in cold-stressed transgenic plants”
t0“Cucumber”
Claim · model-written summary
Cold stressed transgenic plants had higher glutathione reductase activity.
- Taxon
- cucumberCucumis sativust0
- Treatment
- cold-stressed transgenic plantse0
- Outcome
- glutathione reductase (GR) activitiese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glutathione reductase (GR) activities increased in cold-stressed transgenic plants”
t0“Cucumber”
Claim · model-written summary
Transgenic plants produced less ROS, OFR, and H2O2.
- Taxon
- cucumberCucumis sativust0
- Treatment
- transgenic plantse0
- Outcome
- reactive oxygen species (ROS), Oxygen free radical (OFR) and H2O2, productione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The reactive oxygen species (ROS), Oxygen free radical (OFR) and H2O2, production, as well as membrane lipid peroxidation (MDA) production decreased in transgenic plants”
t0“Cucumber”
Claim · model-written summary
Transgenic plants had less membrane lipid peroxidation.
- Taxon
- cucumberCucumis sativust0
- Treatment
- transgenic plantse0
- Outcome
- membrane lipid peroxidation (MDA) productione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“membrane lipid peroxidation (MDA) production decreased in transgenic plants”
t0“Cucumber”
The same questions in other papers
cold · molecular: expression · Cucumis sativus (cucumber)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- negative
- no effect
- no effect
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.