Abstract
Standardization of protocol for callus induction and response of callus to different concentrations of 2, 4-D of two sugarcane genotypes (Co 86032, CoM 0265) was established through callus culture using leaf whorl and meristem as an explant. The results showed that meristem as well as leaf whorl explants were suitable for callus induction in sugarcane. MS medium supplemented with 3 mg/l and 4 mg/l of 2, 4-D was found most suitable for callus induction and multiplication in both the varieties. This treatment also took minimum number of days for callus induction and maximum callus induction percentage in both the genotypes. Profuse and good quality callus induction was observed in MS medium + 3 mg/l and 4 mg/l 2, 4-D in both the explants genotypes.
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Claims 7
Claim · model-written summary
Meristem explants were suitable for sugarcane callus induction.
Verbatim
e0“meristem as well as leaf whorl explants were suitable for callus induction in sugarcane”
Claim · model-written summary
Leaf whorl explants were suitable for sugarcane callus induction.
Verbatim
e0“meristem as well as leaf whorl explants were suitable for callus induction in sugarcane”
Claim · model-written summary
MS medium with 3 mg/l 2, 4-D was among the most suitable for callus induction and multiplication in both varieties.
Verbatim
e0“MS medium supplemented with 3 mg/l and 4 mg/l of 2, 4-D was found most suitable for callus induction and multiplication in both the varieties”
t0“sugarcane (Saccharum officinarum l.)”
Claim · model-written summary
MS medium with 4 mg/l 2, 4-D was among the most suitable for callus induction and multiplication in both varieties.
Verbatim
e0“MS medium supplemented with 3 mg/l and 4 mg/l of 2, 4-D was found most suitable for callus induction and multiplication in both the varieties”
t0“sugarcane (Saccharum officinarum l.)”
Claim · model-written summary
The selected treatments minimized days to callus induction.
Verbatim
e0“This treatment also took minimum number of days for callus induction”
t0“sugarcane (Saccharum officinarum l.)”
Claim · model-written summary
The selected treatments maximized callus induction percentage in both genotypes.
Verbatim
e0“maximum callus induction percentage in both the genotypes”
t0“sugarcane (Saccharum officinarum l.)”
Claim · model-written summary
Both explant genotypes produced profuse, good-quality callus on medium with 3 mg/l or 4 mg/l 2, 4-D.
Verbatim
e0“Profuse and good quality callus induction was observed in MS medium + 3 mg/l and 4 mg/l 2, 4-D in both the explants genotypes”
t0“sugarcane (Saccharum officinarum l.)”