Abstract
The reproductive mechanism of Kernel-apricot involves Gametophytic self-incompatibility (GSI) which is under S-allele control. It encodes a polymorphic pistil ribonuclease (S-RNase), which is multiallelic and ideal markers for S-genotypes characterisation. In this study, six S-RNase primers were used to identify theS-genotypes of 10 Kernel-apricot, including three self-design primers and three universal primers. Ten S-alleles were identified in 10 Kernel-apricot through sequencing and comparing in NCBI. Moreover, some Kernel-apricot S-genotypes were confirmed by experiment of fruit-setting ratio and pollen tube growth. In conclusion, S-genotypes information of Kernel-apricot will be helpful for breeders and growers to plan compatible hybridised combination for ensuring effective pollination and high fruit-setting ratio.
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Claims 3
Claim · model-written summary
Ten S-alleles were identified among 10 kernel-apricot accessions.
Verbatim
e0“to identify theS-genotypes of 10 Kernel-apricot”
e1“Ten S-alleles were identified in 10 Kernel-apricot through sequencing and comparing in NCBI”
Claim · model-written summary
Some kernel-apricot S-genotypes were confirmed by fruit-setting ratio and pollen tube growth experiments.
Verbatim
e0“to identify theS-genotypes of 10 Kernel-apricot”
e2“some Kernel-apricot S-genotypes were confirmed by experiment of fruit-setting ratio and pollen tube growth”
Claim · model-written summary
S-genotype information can help breeders and growers plan compatible hybridised combinations for effective pollination and high fruit-setting ratio.
Verbatim
e3“S-genotypes information of Kernel-apricot will be helpful for breeders and growers to plan compatible hybridised combination for ensuring effective pollination and high fruit-setting ratio”