Abstract
In plants, the mitogen-activated protein kinase (MAPK) cascades are the central signaling pathways of the complicated defense network triggered by the perception of pathogen-associated molecular patterns to repel pathogens. The Arabidopsis thaliana MAPK phosphatase 1 (AtMKP1) negatively regulates the activation of MAPKs. Recently, the AtMKP1 homolog of Nicotiana benthamiana (NbMKP1) was found in association with the Bamboo mosaic virus (BaMV) replication complex. This study aimed to investigate the role of NbMKP1 in BaMV multiplication in N. benthamiana. Silencing of NbMKP1 increased accumulations of the BaMV-encoded proteins and the viral genomic RNA, although the same condition reduced the infectivity of Pseudomonas syringae pv. tomato DC3000 in N. benthamiana. On the other hand, overexpression of NbMKP1 decreased the BaMV coat protein accumulation in a phosphatase activity-dependent manner in protoplasts. NbMKP1 also negatively affected the in vitro RNA polymerase activity of the BaMV replication complex. Collectively, the activity of NbMKP1 seems to reduce BaMV multiplication, inconsistent with the negatively regulatory role of MKP1 in MAPK cascades in terms of warding off fungal and bacterial invasion. In addition, silencing of NbMKP1 increased the accumulation of Foxtail mosaic virus but decreased Potato virus X. The discrepant effects exerted by NbMKP1 on different pathogens foresee the difficulty to develop plants with broad-spectrum resistance through genetically manipulating a single player in MAPK cascades.
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Claims 7
Claim · model-written summary
Silencing NbMKP1 increased BaMV protein accumulation.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Treatment
- Silencing of NbMKP1e0
- Interactor
- BaMVe0pathogen
- Gene
- NbMKP1e0
- Outcome
- BaMV-encoded proteinse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of NbMKP1 increased accumulations of the BaMV-encoded proteins and the viral genomic RNA”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbMKP1 increased BaMV genomic RNA.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of NbMKP1e0
- Interactor
- BaMVe0pathogen
- Gene
- NbMKP1e0
- Outcome
- viral genomic RNAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of NbMKP1 increased accumulations of the BaMV-encoded proteins and the viral genomic RNA”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbMKP1 reduced Pseudomonas infectivity.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- the same conditione0
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e0pathogen
- Outcome
- infectivity of Pseudomonas syringae pv. tomato DC3000e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the same condition reduced the infectivity of Pseudomonas syringae pv. tomato DC3000 in N. benthamiana”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbMKP1 overexpression decreased BaMV coat protein accumulation in protoplasts.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- protoplastse0
- Treatment
- overexpression of NbMKP1e0
- Interactor
- BaMVe0pathogen
- Gene
- NbMKP1e0
- Outcome
- BaMV coat protein accumulatione0
- Direction
- decreasede0
- Qualifier
- phosphatase activity-dependente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of NbMKP1 decreased the BaMV coat protein accumulation in a phosphatase activity-dependent manner in protoplasts”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbMKP1 negatively affected BaMV replication-complex RNA polymerase activity in vitro.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbMKP1e0
- Interactor
- BaMVe0pathogen
- Gene
- NbMKP1e0
- Outcome
- in vitro RNA polymerase activity of the BaMV replication complexe0
- Direction
- negatively affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbMKP1 also negatively affected the in vitro RNA polymerase activity of the BaMV replication complex”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbMKP1 increased Foxtail mosaic virus accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silencing of NbMKP1e0
- Interactor
- Foxtail mosaic virusFoxtail mosaic viruse0pathogen
- Gene
- NbMKP1e0
- Outcome
- accumulation of Foxtail mosaic viruse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of NbMKP1 increased the accumulation of Foxtail mosaic virus”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbMKP1 decreased Potato virus X accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silencing of NbMKP1e0
- Interactor
- Potato virus XPotato virus Xe0pathogen
- Gene
- NbMKP1e0
- Outcome
- Potato virus Xe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of NbMKP1 increased the accumulation of Foxtail mosaic virus but decreased Potato virus X”
t0“Nicotiana benthamiana”
The same questions in other papers
loss of function · Potato virus X · Nicotiana benthamianaPositive and negative results
7 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
- negative
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.