Abstract
Hypersensitive response-related programmed cell death (PCD) has been extensively analyzed in various plant-virus interactions. However, little is known about the changes in gene expression and phytohormone levels associated with cell death caused by compatible viruses. The synergistic interaction of Potato virus X (PVX) with a number of Potyvirus spp. results in increased symptoms that lead to systemic necrosis (SN) in Nicotiana benthamiana. Here, we show that SN induced by a PVX recombinant virus expressing a potyviral helper component-proteinase (HC-Pro) gene is associated with PCD. We have also compared transcriptomic and hormonal changes that occur in response to a compatible synergistic virus interaction that leads to SN, a systemic incompatible interaction conferred by the Tobacco mosaic virus-resistance gene N, and a PCD response conditioned by depletion of proteasome function. Our analysis indicates that the SN response clusters with the incompatible response by the similarity of their overall gene expression profiles. However, the expression profiles of both defense-related genes and hormone-responsive genes, and also the relative accumulation of several hormones in response to SN, relate more closely to the response to depletion of proteasome function than to that elicited by the incompatible interaction. This suggests a potential contribution of proteasome dysfunction to the increased pathogenicity observed in PVX-Potyvirus mixed infections. Furthermore, silencing of coronatine insensitive 1, a gene involved in jasmonate perception, in N. benthamiana accelerated cell death induced by PVX expressing HC-Pro.
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Claims 6
Claim · model-written summary
PVX expressing HC-Pro induced systemic necrosis associated with programmed cell death.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Interactor
- PVXPotato virus Xe0pathogen
- Gene
- potyviral helper component-proteinase (HC-Pro) genee0
- Outcome
- PCDe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SN induced by a PVX recombinant virus expressing a potyviral helper component-proteinase (HC-Pro) gene is associated with PCD”
t0“Nicotiana benthamiana”
Claim · model-written summary
Systemic necrosis and the incompatible response clustered by overall gene expression profiles.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- similarity of their overall gene expression profilese0
- Comparator
- incompatible responsee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the SN response clusters with the incompatible response by the similarity of their overall gene expression profiles”
t0“Nicotiana benthamiana”
Claim · model-written summary
Defense and hormone responsive gene profiles during systemic necrosis were closer to proteasome depletion than to incompatible infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- expression profiles of both defense-related genes and hormone-responsive genese0
- Direction
- more closelye0
- Comparator
- response to depletion of proteasome functione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression profiles of both defense-related genes and hormone-responsive genes, and also the relative accumulation of several hormones in response to SN, relate more closely to the response to depletion of proteasome function than to that elicited by the incompatible interaction”
t0“Nicotiana benthamiana”
Claim · model-written summary
Hormone accumulation during systemic necrosis was closer to proteasome depletion than to the incompatible interaction.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- relative accumulation of several hormonese0
- Direction
- more closelye0
- Comparator
- response to depletion of proteasome functione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the relative accumulation of several hormones in response to SN, relate more closely to the response to depletion of proteasome function than to that elicited by the incompatible interaction”
t0“Nicotiana benthamiana”
Claim · model-written summary
The authors suggest proteasome dysfunction may contribute to pathogenicity in mixed PVX-Potyvirus infections.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- PVX-Potyviruse0pathogen
- Outcome
- increased pathogenicitye0
- Qualifier
- proteasome dysfunctione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potential contribution of proteasome dysfunction to the increased pathogenicity observed in PVX-Potyvirus mixed infections”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing coronatine insensitive 1 accelerated cell death induced by PVX expressing HC-Pro.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- silencing of coronatine insensitive 1e0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- coronatine insensitive 1e0
- Outcome
- cell death induced by PVX expressing HC-Proe0
- Direction
- acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of coronatine insensitive 1, a gene involved in jasmonate perception, in N. benthamiana accelerated cell death induced by PVX expressing HC-Pro”
The same questions in other papers
loss of function · Potato virus X · Nicotiana benthamianaPositive and negative results
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- positive
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