Abstract
Virus infections in plants cause changes in host gene expression that are common to other environmental stresses. In this work, we found extensive overlap in the transcriptional responses between Arabidopsis thaliana plants infected with Tobacco rattle virus (TRV) and plants undergoing senescence. This is exemplified by the up-regulation during infection of several senescence-associated Dark-inducible (DIN) genes, including AtDIN1 (Senescence 1, SEN1), AtDIN6 (Asparagine synthetase 1, AtASN1) and AtDIN11. DIN1, DIN6 and DIN11 homologues were also activated in Nicotiana benthamiana in response to TRV and Potato virus X (PVX) infection. Reduced TRV levels in RNA interference (RNAi) lines targeting AtDIN11 indicate that DIN11 is an important modulator of susceptibility to TRV in Arabidopsis. Furthermore, low accumulation of TRV in Arabidopsis protoplasts from RNAi lines suggests that AtDIN11 supports virus multiplication in this species. The effect of DIN6 on virus accumulation was negligible in Arabidopsis, perhaps as a result of gene or functional redundancy. However, TRV-induced silencing of NbASN, the DIN6 homologue in N. benthamiana, compromises TRV and PVX accumulation in systemically infected leaves. Interestingly, NbASN inactivation correlates with the appearance of morphological defects in infected leaves. We found that DIN6 and DIN11 regulate virus multiplication in a step prior to the activation of plant defence responses. We hypothesize on the possible roles of DIN6 and DIN11 during virus infection.
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Claims 11
Claim · model-written summary
Arabidopsis TRV infection and senescence had extensive overlapping transcriptional responses.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Interactor
- Tobacco rattle virus (TRV)Tobacco rattle viruse0pathogen
- Outcome
- extensive overlap in the transcriptional responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extensive overlap in the transcriptional responses between Arabidopsis thaliana plants infected with Tobacco rattle virus (TRV) and plants undergoing senescence”
Claim · model-written summary
AtDIN1, AtDIN6 and AtDIN11 were upregulated during infection.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Interactor
- Tobacco rattle virus (TRV)Tobacco rattle viruse1pathogen
- Gene
- AtDIN1 (Senescence 1, SEN1), AtDIN6 (Asparagine synthetase 1, AtASN1) and AtDIN11e0
- Outcome
- up-regulation during infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“up-regulation during infection of several senescence-associated Dark-inducible (DIN) genes, including AtDIN1 (Senescence 1, SEN1), AtDIN6 (Asparagine synthetase 1, AtASN1) and AtDIN11”
e1“Arabidopsis thaliana plants infected with Tobacco rattle virus (TRV)”
Claim · model-written summary
DIN homologues were activated in N. benthamiana by TRV.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Interactor
- TRVTobacco rattle viruse0pathogen
- Gene
- DIN1, DIN6 and DIN11 homologuese0
- Outcome
- activatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DIN1, DIN6 and DIN11 homologues were also activated in Nicotiana benthamiana in response to TRV and Potato virus X (PVX) infection”
Claim · model-written summary
DIN homologues were activated in N. benthamiana by PVX.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Interactor
- Potato virus X (PVX)Potato virus Xe0pathogen
- Gene
- DIN1, DIN6 and DIN11 homologuese0
- Outcome
- activatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DIN1, DIN6 and DIN11 homologues were also activated in Nicotiana benthamiana in response to TRV and Potato virus X (PVX) infection”
Claim · model-written summary
AtDIN11 RNAi reduced TRV levels in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- RNA interference (RNAi) lines targeting AtDIN11e0
- Interactor
- TRVTobacco rattle viruse0pathogen
- Gene
- AtDIN11e0
- Outcome
- TRV levelse0
- Direction
- Reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduced TRV levels in RNA interference (RNAi) lines targeting AtDIN11 indicate that DIN11 is an important modulator of susceptibility to TRV in Arabidopsis”
Claim · model-written summary
TRV accumulation was low in AtDIN11 RNAi Arabidopsis protoplasts.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- RNAi linese0
- Interactor
- TRVTobacco rattle viruse0pathogen
- Gene
- AtDIN11e0
- Outcome
- accumulation of TRVe0
- Direction
- lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low accumulation of TRV in Arabidopsis protoplasts from RNAi lines suggests that AtDIN11 supports virus multiplication in this species”
Claim · model-written summary
DIN6 had negligible effect on virus accumulation in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- DIN6e0
- Outcome
- effect of DIN6 on virus accumulation was negligiblee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of DIN6 on virus accumulation was negligible in Arabidopsis”
Claim · model-written summary
NbASN silencing compromised TRV accumulation in N. benthamiana leaves.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- TRV-induced silencing of NbASNe0
- Interactor
- TRVTobacco rattle viruse0pathogen
- Gene
- NbASNe0
- Outcome
- TRV and PVX accumulatione0
- Direction
- compromisese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TRV-induced silencing of NbASN, the DIN6 homologue in N. benthamiana, compromises TRV and PVX accumulation in systemically infected leaves”
Claim · model-written summary
NbASN silencing compromised PVX accumulation in N. benthamiana leaves.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- TRV-induced silencing of NbASNe0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- NbASNe0
- Outcome
- PVX accumulatione0
- Direction
- compromisese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TRV-induced silencing of NbASN, the DIN6 homologue in N. benthamiana, compromises TRV and PVX accumulation in systemically infected leaves”
Claim · model-written summary
NbASN inactivation correlated with morphological defects in infected leaves.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Gene
- NbASNe0
- Outcome
- appearance of morphological defects in infected leavese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbASN inactivation correlates with the appearance of morphological defects in infected leaves”
e1“N. benthamiana”
Claim · model-written summary
DIN6 and DIN11 regulated virus multiplication before plant defence activation.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- DIN6 and DIN11e0
- Outcome
- virus multiplicatione0
- Qualifier
- prior to the activation of plant defence responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DIN6 and DIN11 regulate virus multiplication in a step prior to the activation of plant defence responses”
e1“in Arabidopsis protoplasts from RNAi lines”
e2“N. benthamiana”
The same questions in other papers
loss of function · Tobacco rattle virus · Nicotiana benthamianaPositive and negative results
1 in the opposite direction
- negative
loss of function · Potato virus X · Nicotiana benthamianaPositive and negative results
7 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
- negative
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.