Abstract
Potato virus X coat protein (PVXCP) is, through communication with host proteins, involved in processes such as virus movement and symptom development. Here, we report that PVXCP also interacts with the precursor of plastocyanin, a protein involved in photosynthesis, both in vitro and in vivo. Yeast two-hybrid analysis indicated that PVXCP interacted with only the plastocyanin transit peptide. In subsequent bimolecular fluorescence complementation assays, both proteins were collocated within chloroplasts. Western blot analyses of chloroplast fractions showed that PVXCP could be detected in the envelope, stroma, and lumen fractions. Transmission electron microscopy demonstrated that grana were dilated in PVX-infected Nicotiana benthamiana. Furthermore, virus-induced gene silencing of plastocyanin by prior infection of N. benthamiana using a Tobacco rattle virus vector reduced the severity of symptoms that developed following subsequent PVX infection as well as the accumulation of PVXCP in isolated chloroplasts. However, PVXCP could not be detected in pea chloroplasts in an in vitro re-uptake assay using the plastocyanin precursor protein. Taken together, these data suggest that PVXCP interacts with the plastocyanin precursor protein and that silencing the expression of this protein leads to reduced PVXCP accumulation in chloroplasts and ameliorates symptom severity in host plants.
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Claims 7
Claim · model-written summary
PVX coat protein interacted with the plastocyanin precursor and its transit peptide.
- Gene
- plastocyanin transit peptidee0
- Outcome
- PVXCP interacted with only the plastocyanin transit peptidee0
Not reportedTaxon
Verbatim
e0“PVXCP interacted with only the plastocyanin transit peptide.”
Claim · model-written summary
PVX coat protein and plastocyanin precursor colocalized in chloroplasts.
Not reportedTaxon
Verbatim
e0“both proteins were collocated within chloroplasts.”
Claim · model-written summary
PVX coat protein was detected in chloroplast envelope, stroma and lumen.
- Outcome
- PVXCP could be detected in the envelope, stroma, and lumen fractionse0
Not reportedTaxon
Verbatim
e0“PVXCP could be detected in the envelope, stroma, and lumen fractions.”
Claim · model-written summary
PVX infection dilated grana in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Interactor
- PVXPotato virus Xe0pathogen
- Outcome
- grana were dilatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“grana were dilated in PVX-infected Nicotiana benthamiana.”
Claim · model-written summary
Plastocyanin silencing reduced symptom severity following PVX infection.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- virus-induced gene silencing of plastocyanine0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- plastocyanine0
- Outcome
- severity of symptomse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“virus-induced gene silencing of plastocyanin by prior infection of N. benthamiana using a Tobacco rattle virus vector reduced the severity of symptoms that developed following subsequent PVX infection”
Claim · model-written summary
Plastocyanin silencing reduced PVX coat protein accumulation in chloroplasts.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- virus-induced gene silencing of plastocyanine0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- plastocyanine0
- Outcome
- accumulation of PVXCP in isolated chloroplastse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“virus-induced gene silencing of plastocyanin by prior infection of N. benthamiana using a Tobacco rattle virus vector reduced the severity of symptoms that developed following subsequent PVX infection as well as the accumulation of PVXCP in isolated chloroplasts.”
Claim · model-written summary
PVX coat protein was undetectable in pea chloroplasts in an in vitro re-uptake assay.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- chloroplastse0
- Outcome
- PVXCP could not be detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVXCP could not be detected in pea chloroplasts in an in vitro re-uptake assay using the plastocyanin precursor protein.”
The same questions in other papers
loss of function · Potato virus X · Nicotiana benthamianaPositive and negative results
7 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
- negative
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.