Abstract
Stomata on the plant epidermis control gas and water exchange and are formed by MAPK-dependent processes. Although the contribution of MAP KINASE3 (MPK3) and MPK6 (MPK3/MPK6) to the control of stomatal patterning and differentiation in Arabidopsis (Arabidopsis thaliana) has been examined extensively, how they are inactivated and regulate distinct stages of stomatal development is unknown. Here, we identify a dual-specificity phosphatase, MAP KINASE PHOSPHATASE1 (MKP1), which promotes stomatal cell fate transition by controlling MAPK activation at the early stage of stomatal development. Loss of function of MKP1 creates clusters of small cells that fail to differentiate into stomata, resulting in the formation of patches of pavement cells. We show that MKP1 acts downstream of YODA (a MAPK kinase kinase) but upstream of MPK3/MPK6 in the stomatal signaling pathway and that MKP1 deficiency causes stomatal signal-induced MAPK hyperactivation in vivo. By expressing MKP1 in the three discrete cell types of stomatal lineage, we further identified that MKP1-mediated deactivation of MAPKs in early stomatal precursor cells directs cell fate transition leading to stomatal differentiation. Together, our data reveal the important role of MKP1 in controlling MAPK signaling specificity and cell fate decision during stomatal development.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
MKP1 promotes early stomatal cell fate transition through MAPK activation control.
- Taxon
- Arabidopsis
Arabidopsise1 - Organ / stage
- early stage of stomatal developmente0
- Gene
- MAP KINASE PHOSPHATASE1 (MKP1)e0
- Outcome
- stomatal cell fate transitione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we identify a dual-specificity phosphatase, MAP KINASE PHOSPHATASE1 (MKP1), which promotes stomatal cell fate transition by controlling MAPK activation at the early stage of stomatal development.”
e1“in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MKP1 loss creates small cell clusters that fail to become stomata.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Loss of function of MKP1e0
- Gene
- MKP1e0
- Outcome
- clusters of small cells that fail to differentiate into stomatae0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of function of MKP1 creates clusters of small cells that fail to differentiate into stomata, resulting in the formation of patches of pavement cells.”
e1“in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MKP1 loss results in patches of pavement cells.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Loss of function of MKP1e0
- Gene
- MKP1e0
- Outcome
- patches of pavement cellse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss of function of MKP1 creates clusters of small cells that fail to differentiate into stomata, resulting in the formation of patches of pavement cells.”
e1“in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MKP1 acts downstream of YODA and upstream of MPK3/MPK6.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- MKP1e0
- Outcome
- stomatal signaling pathwaye0
- Qualifier
- downstream of YODA (a MAPK kinase kinase) but upstream of MPK3/MPK6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We show that MKP1 acts downstream of YODA (a MAPK kinase kinase) but upstream of MPK3/MPK6 in the stomatal signaling pathway and that MKP1 deficiency causes stomatal signal-induced MAPK hyperactivation in vivo.”
e1“in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MKP1 deficiency causes stomatal signal-induced MAPK hyperactivation.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- MKP1 deficiencye0
- Gene
- MKP1e0
- Outcome
- stomatal signal-induced MAPK hyperactivatione0
- Direction
- hyperactivatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We show that MKP1 acts downstream of YODA (a MAPK kinase kinase) but upstream of MPK3/MPK6 in the stomatal signaling pathway and that MKP1 deficiency causes stomatal signal-induced MAPK hyperactivation in vivo.”
e1“in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MKP1-mediated MAPK deactivation in early precursor cells directs stomatal differentiation.
- Taxon
- ArabidopsisArabidopsise1
- Organ / stage
- early stomatal precursor cellse0
- Gene
- MKP1e0
- Outcome
- cell fate transition leading to stomatal differentiatione0
- Qualifier
- deactivation of MAPKse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By expressing MKP1 in the three discrete cell types of stomatal lineage, we further identified that MKP1-mediated deactivation of MAPKs in early stomatal precursor cells directs cell fate transition leading to stomatal differentiation.”
e1“in Arabidopsis (Arabidopsis thaliana)”
The same questions in other papers
loss of function · physiology: gas exchange · ArabidopsisPositive and negative results
4 papers in the same direction as this paper · 20 in the opposite direction
- negative
- negative
- negative
21 papers more in this group
See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.