Abstract
Glutathione is the most important part of a plant’s antioxidant defense system. Two enzymes are involved in cellular glutathione biosynthesis: glutamylcysteine ligase and glutathione synthetase, of which the latter catalyzes the transfer of glycine to the glutamylcysteine dipeptide. Transgenic plants with an elevated expression level of the glutathione synthetase genes are known to be tolerant to heavy metals. However, our knowledge on their tolerance to other types of the abiotic stress is insufficient. The goal of this study is to produce transgenic tobacco plants with a constitutive expression of the glutathione synthetase gene BnGSH from rapeseed and to estimate their growth parameters under the normal conditions, as well as under the effects of salt, drought, and cold stresses. We generate 17 lines of transgenic plants with the rapeseed BnGSH gene under the control of the 35S promoter by agrobacterium-mediated transformation. The transgene presence is confirmed by the PCR and histochemical analysis of the activity of the GUS reporter gene. Twelve lines with the highest expression level of the BnGSH gene are chosen based on the results of RT-PCR. The following morphological parameters are measured: stem height, leaf area, flower length, fresh and dry weights of shoots, and root length. Some transgenic plants demonstrated increased productivity both under normal conditions and under the effect of a high salinity stress. However, no change in the tolerance to drought and cold was observed in the transgenic plants.
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Claims 7
Claim · model-written summary
Seventeen tobacco lines carrying rapeseed BnGSH under a 35S promoter were generated.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Gene
- BnGSHe0
- Outcome
- lines of transgenic plantse0
- Value
- 17 linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We generate 17 lines of transgenic plants with the rapeseed BnGSH gene under the control of the 35S promoter”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
The transgene was confirmed by PCR and GUS activity analysis.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- GUS reporter genee0
- Outcome
- transgene presence is confirmede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgene presence is confirmed by the PCR and histochemical analysis of the activity of the GUS reporter gene”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
Twelve transgenic lines showed the highest BnGSH expression by RT-PCR.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- BnGSH genee0
- Outcome
- highest expression levele0
- Value
- Twelve linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twelve lines with the highest expression level of the BnGSH gene are chosen based on the results of RT-PCR”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
Some transgenic tobacco plants had increased productivity under normal conditions.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Constitutive Expression of the Rapeseed Glutathione Synthetase Gene BnGSHt0
- Outcome
- productivitye0
- Direction
- increasede0
- Qualifier
- normal conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some transgenic plants demonstrated increased productivity both under normal conditions and under the effect of a high salinity stress”
t0“Transgenic Tobacco Plants with a Constitutive Expression of the Rapeseed Glutathione Synthetase Gene BnGSH”
Claim · model-written summary
Some transgenic tobacco plants had increased productivity under high salinity stress.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- high salinity stresse0
- Gene
- BnGSHt0
- Outcome
- productivitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some transgenic plants demonstrated increased productivity both under normal conditions and under the effect of a high salinity stress”
t0“Transgenic Tobacco Plants with a Constitutive Expression of the Rapeseed Glutathione Synthetase Gene BnGSH”
Claim · model-written summary
Transgenic tobacco plants showed no change in drought tolerance.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- droughte0
- Outcome
- tolerance to droughte0
- Direction
- no changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no change in the tolerance to drought and cold was observed in the transgenic plants”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
Transgenic tobacco plants showed no change in cold tolerance.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- colde0
- Outcome
- tolerance to drought and colde0
- Direction
- no changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no change in the tolerance to drought and cold was observed in the transgenic plants”
t0“Transgenic Tobacco Plants”