Abstract
The transgenic technology using a single gene has been widely used for crop improvement. But the transgenic pyramiding of multiple genes, a promising alternative especially for enhancing complexly inherited abiotic stress tolerance, has received little attention. Here, we developed and evaluated transgenic rice lines with a single Salt Responsive Protein 3-1 (SaSRP3-1) gene as well as pyramids with two-genes SaSRP3-1 and Vacuolar H+-ATPase subunit c1 (SaVHAc1) derived from a halophyte grass Spartina alterniflora L. for salt tolerance at seedling, vegetative, and reproductive stages. The overexpression of this novel gene SaSRP3-1 resulted in significantly better growth of E. coli with the recombinant plasmid under 600 mM NaCl stress condition compared with the control. During early seedling and vegetative stages, the single gene and pyramided transgenic rice plants showed enhanced tolerance to salt stress with minimal wilting and drying symptoms, improved shoot and root growth, and significantly higher chlorophyll content, relative water content, and K+/Na+ ratio than the control plants. The salt stress screening during reproductive stage revealed that the transgenic plants with single gene and pyramids had better grain filling, whereas the pyramided plants showed significantly higher grain yield and higher grain weight compared to control plants. Our study demonstrated transgenic pyramiding as a viable approach to achieve higher level of salt tolerance in crop plants.
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Claims 7
Claim · model-written summary
Overexpression of SaSRP3-1 significantly improved E. coli growth under 600 mM NaCl compared with the control.
- Taxon
- rice
Oryza sativat1 - Treatment
- overexpression of this novel gene SaSRP3-1e3
- Gene
- SaSRP3-1e3
- Outcome
- growth of E. colie3
- Direction
- significantly bettere3
- Qualifier
- 600 mM NaCl stress conditione3
- Comparator
- controle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Enhances Salt Tolerance in Rice”
e3“The overexpression of this novel gene SaSRP3-1 resulted in significantly better growth of E. coli with the recombinant plasmid under 600 mM NaCl stress condition compared with the control”
Claim · model-written summary
Single-gene and pyramided transgenic rice showed enhanced tolerance to salt stress compared with control plants at seedling and vegetative stages.
- Taxon
- riceOryza sativat1
- Material
- single gene and pyramided transgenic rice plantse4
- Organ / stage
- early seedling and vegetative stagese4
- Treatment
- salt stresse4
- Outcome
- tolerance to salt stresse4
- Direction
- enhancede4
- Comparator
- control plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Enhances Salt Tolerance in Rice”
e4“During early seedling and vegetative stages, the single gene and pyramided transgenic rice plants showed enhanced tolerance to salt stress with minimal wilting and drying symptoms, improved shoot and root growth, and significantly higher chlorophyll content, relative water content, and K+/Na+ ratio than the control plants”
Claim · model-written summary
Transgenic rice under salt stress showed improved shoot and root growth compared with control plants at seedling and vegetative stages.
- Taxon
- riceOryza sativat1
- Material
- single gene and pyramided transgenic rice plantse4
- Organ / stage
- early seedling and vegetative stagese4
- Treatment
- salt stresse4
- Outcome
- shoot and root growthe4
- Direction
- improvede4
- Comparator
- control plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Enhances Salt Tolerance in Rice”
e4“During early seedling and vegetative stages, the single gene and pyramided transgenic rice plants showed enhanced tolerance to salt stress with minimal wilting and drying symptoms, improved shoot and root growth, and significantly higher chlorophyll content, relative water content, and K+/Na+ ratio than the control plants”
Claim · model-written summary
Transgenic rice under salt stress had significantly higher chlorophyll content, relative water content and K+/Na+ ratio than control plants.
- Taxon
- riceOryza sativat1
- Material
- single gene and pyramided transgenic rice plantse4
- Organ / stage
- early seedling and vegetative stagese4
- Treatment
- salt stresse4
- Outcome
- chlorophyll content, relative water content, and K+/Na+ ratioe4
- Direction
- significantly highere4
- Comparator
- control plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Enhances Salt Tolerance in Rice”
e4“During early seedling and vegetative stages, the single gene and pyramided transgenic rice plants showed enhanced tolerance to salt stress with minimal wilting and drying symptoms, improved shoot and root growth, and significantly higher chlorophyll content, relative water content, and K+/Na+ ratio than the control plants”
Claim · model-written summary
Under salt stress at the reproductive stage, single-gene and pyramided transgenic plants had better grain filling than control plants.
- Taxon
- riceOryza sativat1
- Material
- transgenic plants with single gene and pyramidse5
- Organ / stage
- reproductive stagee5
- Treatment
- salt stresse5
- Outcome
- grain fillinge5
- Direction
- bettere5
- Comparator
- control plantse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Enhances Salt Tolerance in Rice”
e5“The salt stress screening during reproductive stage revealed that the transgenic plants with single gene and pyramids had better grain filling, whereas the pyramided plants showed significantly higher grain yield and higher grain weight compared to control plants”
Claim · model-written summary
Under salt stress the pyramided rice plants had significantly higher grain yield and grain weight than control plants.
- Taxon
- riceOryza sativat1
- Material
- pyramided plantse5
- Organ / stage
- reproductive stagee5
- Treatment
- salt stresse5
- Outcome
- grain yield and higher grain weighte5
- Direction
- significantly highere5
- Comparator
- control plantse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Enhances Salt Tolerance in Rice”
e5“The salt stress screening during reproductive stage revealed that the transgenic plants with single gene and pyramids had better grain filling, whereas the pyramided plants showed significantly higher grain yield and higher grain weight compared to control plants”
Claim · model-written summary
Transgenic pyramiding is a viable approach to achieve a higher level of salt tolerance in rice.
- Taxon
- riceOryza sativat1
- Outcome
- higher level of salt tolerancee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Enhances Salt Tolerance in Rice”
e6“Our study demonstrated transgenic pyramiding as a viable approach to achieve higher level of salt tolerance in crop plants”
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