Abstract
In vitro propagation is important for rapid multiplication of a wide range of nursery crops or medicinal plants, including stevia (Stevia rebaudiana Bertoni). The aim of this study was to investigate the effects of mineral salts on plant growth and development of stevia shoot cultures. Response surface methodology was used to design experiments by varying three factors: nitrogen salts (NH4NO3 and KNO3), mesos salts (CaCl2, KH2PO4 and MgSO4) and minor elements (Zn-Mn-Cu-Co-Mo-B-I-EDTA-chelated iron). The concentrations of each factor were defined as relative concentrations compared to Murashige and Skoog (MS) concentrations (0.5–3.0 × MS). The effects were evaluated of these three factors on plant quality, multiplication, shoot length and leaf numbers. The minor elements were the most significant factors associated with shoot length and leaf numbers. Increasing minor elements above an MS level of 1 × and decreasing nitrogen tended to increase shoot length significantly. Increasing minor elements and nitrogen up to 3 × MS and increasing mesos to 1.5 × MS were required to improve leaf numbers. Two optimized media were compared to MS for growth characteristics, phenolics and antioxidant activity. One of the media was identified as significantly better than MS for growth, low phenolic production and low antioxidant response.
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Claims 8
Claim · model-written summary
Minor elements were the most significant factors associated with shoot length and leaf number.
SteviaNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The minor elements were the most significant factors associated with shoot length and leaf numbers.”
t0“stevia”
Claim · model-written summary
Increasing minor elements above 1 × MS tended to increase shoot length significantly.
- Treatment
- Increasing minor elements above an MS level of 1 ×e0
- Outcome
- shoot lengthe0
- Direction
- increasee0
- Comparator
- 1 ×e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing minor elements above an MS level of 1 × and decreasing nitrogen tended to increase shoot length significantly.”
t0“stevia”
Claim · model-written summary
Decreasing nitrogen tended to increase shoot length significantly.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing minor elements above an MS level of 1 × and decreasing nitrogen tended to increase shoot length significantly.”
t0“stevia”
Claim · model-written summary
Increasing minor elements and nitrogen up to 3 × MS improved leaf numbers.
- Treatment
- Increasing minor elements and nitrogen up to 3 × MSe0
- Outcome
- leaf numberse0
- Direction
- improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing minor elements and nitrogen up to 3 × MS and increasing mesos to 1.5 × MS were required to improve leaf numbers.”
t0“stevia”
Claim · model-written summary
Increasing mesos to 1.5 × MS improved leaf numbers.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing minor elements and nitrogen up to 3 × MS and increasing mesos to 1.5 × MS were required to improve leaf numbers.”
t0“stevia”
Claim · model-written summary
One optimized medium improved growth versus MS.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of the media was identified as significantly better than MS for growth, low phenolic production and low antioxidant response.”
t0“stevia”
Claim · model-written summary
One optimized medium yielded low phenolic production relative to MS.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of the media was identified as significantly better than MS for growth, low phenolic production and low antioxidant response.”
t0“stevia”
Claim · model-written summary
One optimized medium yielded a low antioxidant response relative to MS.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of the media was identified as significantly better than MS for growth, low phenolic production and low antioxidant response.”
t0“stevia”
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nitrogen · growth · SteviaResults agree
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7 papers in the same direction as this paper
- positive
- positive
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.