Abstract
An efficient method for the micro propagation of hyssop (Hyssopus officinalis L.) has been developed. The plants were cultured on Murashige and Skoog's medium supplemented with 6-benzyl-aminopurine, thidiazuron, zeatin and indole-3butyric acid after four weeks of culture. Maximum numbers of shoots, shoot length, fresh weight and dry weight were established at 1.0 mg/L 6-benzyl-aminopurine and 0.1 mg/L indole-3-butyric acid. For induction of root, uniform micro shoots were excised and transferred to the rooting medium (half strength Murashige and Skoog medium macro and microelements) supplemented with 0.1 mg/L indole-3-butyric acid and 20 g/L sucrose. Indole-3-butyric acid increased culture rooting, number of roots and root length more efficiently at 0.1 mg/L after four weeks of culture. The multiple plants were successfully ex vitro adapted with 90% survival. The described protocol allows the establishment of numerous micro propagated plants of H. officinalis. The used growth regulators for hyssop micro propagation influenced plant antioxidant system. Antioxidant defence of micropropagated H. officinalis determined by the activities of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase) resulted in higher shoot formation and increasing of shoot number per explant.
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Claims 7
Claim · model-written summary
Maximum numbers of hyssop shoots, shoot length and fresh weight were obtained at 1.0 mg/L BAP with 0.1 mg/L IBA.
- Taxon
- Hyssopus officinalis L.
Dracocephalum officinalet0 - Treatment
- 1.0 mg/L 6-benzyl-aminopurine and 0.1 mg/L indole-3-butyric acide1
- Outcome
- numbers of shoots, shoot length, fresh weighte1
- Direction
- Maximume1
- Setting
- in vitrot0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro propagated hyssop (Hyssopus officinalis L.)”
e1“Maximum numbers of shoots, shoot length, fresh weight and dry weight were established at 1.0 mg/L 6-benzyl-aminopurine and 0.1 mg/L indole-3-butyric acid”
Claim · model-written summary
Maximum dry weight of hyssop was obtained at 1.0 mg/L BAP with 0.1 mg/L IBA.
- Taxon
- Hyssopus officinalis L.Dracocephalum officinalet0
- Treatment
- 1.0 mg/L 6-benzyl-aminopurine and 0.1 mg/L indole-3-butyric acide1
- Outcome
- dry weighte1
- Direction
- Maximume1
- Setting
- in vitrot0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro propagated hyssop (Hyssopus officinalis L.)”
e1“Maximum numbers of shoots, shoot length, fresh weight and dry weight were established at 1.0 mg/L 6-benzyl-aminopurine and 0.1 mg/L indole-3-butyric acid”
Claim · model-written summary
Indole-3-butyric acid at 0.1 mg/L increased rooting, root number and root length of in vitro hyssop shoots.
- Taxon
- Hyssopus officinalis L.Dracocephalum officinalet0
- Treatment
- Indole-3-butyric acide2
- Outcome
- culture rooting, number of roots and root lengthe2
- Direction
- increasede2
- Qualifier
- 0.1 mg/L after four weeks of culturee2
- Setting
- in vitrot0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro propagated hyssop (Hyssopus officinalis L.)”
e2“Indole-3-butyric acid increased culture rooting, number of roots and root length more efficiently at 0.1 mg/L after four weeks of culture”
Claim · model-written summary
Micropropagated hyssop plants had 90% survival after ex vitro adaptation.
- Taxon
- Hyssopus officinalis L.Dracocephalum officinalet0
- Outcome
- survivale3
- Value
- 90%e3
- Setting
- ex vitroe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro propagated hyssop (Hyssopus officinalis L.)”
e3“The multiple plants were successfully ex vitro adapted with 90% survival”
Claim · model-written summary
Growth regulators used in hyssop micropropagation influenced the plant antioxidant system.
- Taxon
- Hyssopus officinalis L.Dracocephalum officinalet0
- Treatment
- growth regulatorse4
- Outcome
- plant antioxidant systeme4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro propagated hyssop (Hyssopus officinalis L.)”
e4“The used growth regulators for hyssop micro propagation influenced plant antioxidant system”
Claim · model-written summary
Antioxidant enzyme activities in micropropagated hyssop were associated with higher shoot formation.
- Taxon
- H. officinalisDracocephalum officinalee5
- Gene
- superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidasee5
- Outcome
- shoot formatione5
- Direction
- highere5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro propagated hyssop (Hyssopus officinalis L.)”
e5“Antioxidant defence of micropropagated H. officinalis determined by the activities of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase) resulted in higher shoot formation and increasing of shoot number per explant”
Claim · model-written summary
Antioxidant enzyme activities in micropropagated hyssop were associated with increasing shoot number per explant.
- Taxon
- H. officinalisDracocephalum officinalee5
- Gene
- superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidasee5
- Outcome
- shoot number per explante5
- Direction
- increasinge5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro propagated hyssop (Hyssopus officinalis L.)”
e5“Antioxidant defence of micropropagated H. officinalis determined by the activities of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase) resulted in higher shoot formation and increasing of shoot number per explant”
The same questions in other papers
indole-3-butyric acid · growth · Dracocephalum officinale (hyssop)Results agree
1 paper in the same direction as this paper
- positive
N-benzyladenine · growth · Dracocephalum officinale (hyssop)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.