Abstract
Tospovirus is a tripartite negative stranded RNA virus and is considered as one of the most devastating plant viruses. Successful virus infection in plant requires many host factors. To date, very few host factors have been identified as important in Tospovirus infection in plants. We reported earlier that NSm protein encoded by Tomato spotted wilt virus (TSWV), a type species of the genus Orthotospovirus, plays critical roles in viral cell-to-cell and long-distance movement. In this study, we determined that molecular co-chaperone NbSGT1 interacted with TSWV NSm in Nicotiana benthamiana. TSWV infection significantly upregulated the expression of NbSGT1 gene and transient overexpression of NbSGT1 in N. benthamiana leaves accelerated TSWV infection. In contrast, silencing the NbSGT1 gene expression using a virus-induced gene silencing (VIGS) approach strongly inhibited TSWV NSm cell-to-cell movement, as well as TSWV local and systemic infection in N. benthamiana plants. Furthermore, NbSGT1 was found to regulate the infection of both American and Euro/Asia type tospoviruses in N. benthamiana plant. Collectively, our findings presented in this paper and the results published previously indicated that molecular co-chaperone NbSGT1 plays important roles in modulating both positive stranded and tripartite negative stranded RNA virus infection in plants.
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Claims 5
Claim · model-written summary
NbSGT1 interacted with TSWV NSm.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Interactor
- TSWV
Orthotospovirus tomatomaculaee0pathogen - Gene
- NbSGT1e0
- Outcome
- interacted with TSWV NSme0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“molecular co-chaperone NbSGT1 interacted with TSWV NSm in Nicotiana benthamiana.”
Claim · model-written summary
TSWV infection upregulated NbSGT1 expression.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Treatment
- TSWV infectione0
- Interactor
- TSWVOrthotospovirus tomatomaculaee0pathogen
- Outcome
- expression of NbSGT1 genee0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TSWV infection significantly upregulated the expression of NbSGT1 gene”
e1“in N. benthamiana leaves”
Claim · model-written summary
NbSGT1 overexpression accelerated TSWV infection.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- transient overexpression of NbSGT1e0
- Interactor
- TSWVOrthotospovirus tomatomaculaee0pathogen
- Gene
- NbSGT1e0
- Outcome
- TSWV infectione0
- Direction
- acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient overexpression of NbSGT1 in N. benthamiana leaves accelerated TSWV infection.”
Claim · model-written summary
NbSGT1 silencing inhibited NSm cell to cell movement and local and systemic infection.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- silencing the NbSGT1 gene expressione0
- Interactor
- TSWVOrthotospovirus tomatomaculaee0pathogen
- Gene
- NbSGT1e0
- Outcome
- TSWV NSm cell-to-cell movement, as well as TSWV local and systemic infectione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing the NbSGT1 gene expression using a virus-induced gene silencing (VIGS) approach strongly inhibited TSWV NSm cell-to-cell movement, as well as TSWV local and systemic infection in N. benthamiana plants.”
Claim · model-written summary
NbSGT1 regulated infection by American and Euro/Asia type tospoviruses.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- tospoviruses
Orthotospoviruse0pathogen - Gene
- NbSGT1e0
- Outcome
- infection of both American and Euro/Asia type tospovirusese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbSGT1 was found to regulate the infection of both American and Euro/Asia type tospoviruses in N. benthamiana plant.”
The same questions in other papers
loss of function · Orthotospovirus tomatomaculae · Nicotiana benthamianaPositive and negative results
5 in the opposite direction
- negative
- negative
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.