Abstract
Potato is an important crop widely cultivated throughout the world. It is prone to several pathogenic fungi, viruses, and bacteria, which cause severe economic loss every year. Recent advances in plant biotechnology have made it possible to produce resistant cultivars by plant genome editing. This approach allows crop modiications that would be diicult to obtain by conventional breeding techniques. A successful and reproducible plant system requires a responsive in vitro regeneration system. Three Siberian potato cultivars –Kemerovchanin, Tuleevsky, and Sapho – and two control cultivars – Golubizna and Nikulinsky – were chosen for in vitro response tests. The stem explants were excised from in vitro grown plantlets. Their stem explants were incubated on Murashige&Skoog (MS) medium supplemented with 1 mg/L transzeatin, 0.1 mg/L IAA, and 10 mg/L GA 3 with vitamins (medium P1). All the stages of cultivation up to obtaining fullledged regenerant plants were carried out on medium P1. Every two weeks the explants were transferred to fresh nutrient media. All cultivars formed calluses but difered in terms of callus formation and in callus type. With further cultivation on the medium of the same composition, morphogenesis was observed: shoots formed on wound surfaces of the stem explants. Cultivars Tuleevsky, Kemerovchanin, and Golubizna showed high regeneration ability (73–97.7 %), while that of Sapho (63 %) was poor. All the Siberian cultivars were variable with regard to their morphogenic potentials. Root formation was observed in the shoots within 7 days on hormonefree MS. In the control (hormonefree medium), stem explants of all cultivars did not form calluses or develop shoots on the wound surfaces. The protocol described here is simple and eicient. It can be applied to other potato cultivars.
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Claims 10
Claim · model-written summary
All potato cultivars formed calluses, with differences in callus formation and type.
- Taxon
- POTATO
Solanum tuberosumt0 - Outcome
- callusese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All cultivars formed calluses but difered in terms of callus formation and in callus type.”
t0“POTATO”
Claim · model-written summary
Shoots formed on wound surfaces of stem explants during further cultivation.
- Taxon
- POTATOSolanum tuberosumt0
- Organ / stage
- stem explantse0
- Treatment
- medium of the same compositione0
- Outcome
- shoots formede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With further cultivation on the medium of the same composition, morphogenesis was observed: shoots formed on wound surfaces of the stem explants.”
t0“POTATO”
Claim · model-written summary
Tuleevsky showed high regeneration ability within the reported 73–97.7% range.
- Taxon
- POTATOSolanum tuberosumt0
- Material
- Tuleevskye0
- Outcome
- regeneration abilitye0
- Value
- 73–97.7 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultivars Tuleevsky, Kemerovchanin, and Golubizna showed high regeneration ability (73–97.7 %), while that of Sapho (63 %) was poor.”
t0“POTATO”
Claim · model-written summary
Kemerovchanin showed high regeneration ability within the reported 73–97.7% range.
- Taxon
- POTATOSolanum tuberosumt0
- Material
- Kemerovchanine0
- Outcome
- regeneration abilitye0
- Value
- 73–97.7 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultivars Tuleevsky, Kemerovchanin, and Golubizna showed high regeneration ability (73–97.7 %), while that of Sapho (63 %) was poor.”
t0“POTATO”
Claim · model-written summary
Golubizna showed high regeneration ability within the reported 73–97.7% range.
- Taxon
- POTATOSolanum tuberosumt0
- Material
- Golubiznae0
- Outcome
- regeneration abilitye0
- Value
- 73–97.7 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultivars Tuleevsky, Kemerovchanin, and Golubizna showed high regeneration ability (73–97.7 %), while that of Sapho (63 %) was poor.”
t0“POTATO”
Claim · model-written summary
Sapho showed poor regeneration ability of 63%.
- Taxon
- POTATOSolanum tuberosumt0
- Material
- Saphoe0
- Outcome
- regeneration abilitye0
- Value
- 63 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultivars Tuleevsky, Kemerovchanin, and Golubizna showed high regeneration ability (73–97.7 %), while that of Sapho (63 %) was poor.”
t0“POTATO”
Claim · model-written summary
Siberian cultivars varied in morphogenic potential.
- Taxon
- POTATOSolanum tuberosumt0
- Material
- Siberian cultivarse0
- Outcome
- morphogenic potentialse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All the Siberian cultivars were variable with regard to their morphogenic potentials.”
t0“POTATO”
Claim · model-written summary
Shoots formed roots within seven days on hormone-free MS.
- Taxon
- POTATOSolanum tuberosumt0
- Organ / stage
- shootse0
- Treatment
- hormonefree MSe0
- Outcome
- Root formatione0
- Value
- 7 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root formation was observed in the shoots within 7 days on hormonefree MS.”
t0“POTATO”
Claim · model-written summary
On hormone-free control medium, stem explants did not form calluses or shoots.
- Taxon
- POTATOSolanum tuberosumt0
- Organ / stage
- stem explantse0
- Treatment
- hormonefree mediume0
- Outcome
- did not form calluses or develop shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the control (hormonefree medium), stem explants of all cultivars did not form calluses or develop shoots on the wound surfaces.”
t0“POTATO”
Claim · model-written summary
The authors describe the regeneration protocol as simple and efficient and applicable to other cultivars.
- Taxon
- POTATOSolanum tuberosumt0
- Outcome
- simple and eiciente0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protocol described here is simple and eicient. It can be applied to other potato cultivars.”
t0“POTATO”