Abstract
) has a high tolerance to potassium deficiency (LK) stress. The stem is the primary organ responsible for transporting molecules from the underground root system to the aboveground parts of the plant. However, proteomic changes in response to LK stress are largely unknown in alligator weed stems. In this study, we investigated the physiological and proteomic changes in alligator weed stems under LK stress. First, the chlorophyll and soluble protein content and SOD and POD activity were significantly altered after 15 days of LK treatment. The quantitative proteomic analysis suggested that a total of 296 proteins were differentially abundant proteins (DAPs). The functional annotation analysis revealed that LK stress elicited complex proteomic alterations that were involved in oxidative phosphorylation, plant-pathogen interactions, glycolysis/gluconeogenesis, sugar metabolism, and transport in stems. The subcellular locations analysis suggested 104 proteins showed chloroplastic localization, 81 proteins showed cytoplasmic localization and 40 showed nuclear localization. The protein⁻protein interaction analysis revealed that 56 proteins were involved in the interaction network, including 9 proteins involved in the ribosome network and 9 in the oxidative phosphorylation network. Additionally, the expressed changes of 5 DAPs were similar between the proteomic quantification analysis and the PRM-MS analysis, and the expression levels of eight genes that encode DAPs were further verified using an RT-qPCR analysis. These results provide valuable information on the adaptive mechanisms in alligator weed stems under LK stress and facilitate the development of efficient strategies for genetically engineering potassium-tolerant crops.
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Claims 11
Claim · model-written summary
Potassium deficiency significantly altered chlorophyll and soluble protein content and SOD and POD activity after 15 days.
- Taxon
- Alligator Weedt0
- Organ / stage
- Stemst0
- Treatment
- LK treatmente0
- Outcome
- chlorophyll and soluble protein content and SOD and POD activitye0
Not reportedComparatorDirection
Verbatim
t0“Alligator Weed Stems”
e0“chlorophyll and soluble protein content and SOD and POD activity were significantly altered after 15 days of LK treatment”
Claim · model-written summary
Quantitative proteomics identified 296 differentially abundant proteins.
Verbatim
t0“Alligator Weed Stems”
e0“a total of 296 proteins were differentially abundant proteins (DAPs)”
Claim · model-written summary
Potassium deficiency affected proteins involved in several metabolic and transport pathways.
Verbatim
t0“Alligator Weed Stems”
e0“LK stress elicited complex proteomic alterations that were involved in oxidative phosphorylation, plant-pathogen interactions, glycolysis/gluconeogenesis, sugar metabolism, and transport in stems”
Claim · model-written summary
The subcellular analysis localized 104 proteins to chloroplasts.
Verbatim
t0“Alligator Weed Stems”
e0“104 proteins showed chloroplastic localization”
Claim · model-written summary
The subcellular analysis localized 81 proteins to cytoplasm.
Verbatim
t0“Alligator Weed Stems”
e0“81 proteins showed cytoplasmic localization”
Claim · model-written summary
The subcellular analysis localized 40 proteins to nuclei.
Verbatim
t0“Alligator Weed Stems”
e0“40 showed nuclear localization”
Claim · model-written summary
A protein interaction network contained 56 proteins.
Verbatim
t0“Alligator Weed Stems”
e0“56 proteins were involved in the interaction network”
Claim · model-written summary
Nine proteins were in the ribosome network.
Verbatim
t0“Alligator Weed Stems”
e0“9 proteins involved in the ribosome network”
Claim · model-written summary
Nine proteins were in the oxidative phosphorylation network.
Verbatim
t0“Alligator Weed Stems”
e0“9 in the oxidative phosphorylation network”
Claim · model-written summary
Five proteins had similar expression changes by proteomic quantification and PRM-MS.
Verbatim
t0“Alligator Weed Stems”
e0“expressed changes of 5 DAPs were similar between the proteomic quantification analysis and the PRM-MS analysis”
Claim · model-written summary
Expression of eight genes encoding differentially abundant proteins was verified by RT-qPCR.
Verbatim
t0“Alligator Weed Stems”
e0“expression levels of eight genes that encode DAPs were further verified using an RT-qPCR analysis”