Abstract
Leea indica (Vitaceae) is a Southeast Asian medicinal plant. In this study, an ethyl acetate fraction of L. indica leaves was studied for its phytoconstituents using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-microTOF-Q-MS/MS) analysis. A total of 31 compounds of different classes, including benzoic acid derivatives, phenolics, flavonoids, catechins, dihydrochalcones, coumarins, megastigmanes, and oxylipins were identified using LC-MS/MS. Among them, six compounds including gallic acid, methyl gallate, (−)-epigallocatechin-3-O-gallate, myricetin-3-O-rhamnoside, quercetin-3-O-rhamnoside, and 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-β-d-glucopyranoside were isolated and identified by NMR analysis. The LC-MS/MS analysis led to the tentative identification of three novel dihydrochalcones namely 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-rutinoside, 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-glucosylpentoside and 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-(3″-O-galloyl)-β-d-glucopyranoside. The structural identification of novel dihydrochalcones was based on the basic skeleton of the isolated dihydrochalcone, 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-β-d-glucopyranoside and characteristic LC-MS/MS fragmentation patterns. This is the first comprehensive analysis for the identification of compounds from L. indica using LC-MS. A total 24 compounds including three new dihydrochalcones were identified for the first time from the genus Leea.
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Claims 4
Claim · model-written summary
LC-MS/MS identified 31 compounds in L. indica leaves.
- Taxon
- Leea indica
Leea indicat0 - Organ / stage
- Leavest0
- Outcome
- compounds of different classese0
- Value
- 31 compoundse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 31 compounds of different classes, including benzoic acid derivatives, phenolics, flavonoids, catechins, dihydrochalcones, coumarins, megastigmanes, and oxylipins were identified using LC-MS/MS.”
t0“Leea indica (Burm. F.) Merr. Leaves”
Claim · model-written summary
Six named compounds were isolated and identified by NMR.
- Taxon
- Leea indicaLeea indicat0
- Outcome
- gallic acid, methyl gallate, (−)-epigallocatechin-3-O-gallatee0
- Value
- six compoundse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“six compounds including gallic acid, methyl gallate, (−)-epigallocatechin-3-O-gallate, myricetin-3-O-rhamnoside, quercetin-3-O-rhamnoside, and 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-β-d-glucopyranoside were isolated and identified by NMR analysis.”
t0“Leea indica (Burm. F.) Merr. Leaves”
Claim · model-written summary
LC-MS/MS tentatively identified three novel dihydrochalcones.
- Taxon
- Leea indicaLeea indicat0
- Outcome
- three novel dihydrochalconese0
- Qualifier
- tentative identificatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The LC-MS/MS analysis led to the tentative identification of three novel dihydrochalcones namely 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-rutinoside, 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-glucosylpentoside and 4′,6′-dihydroxy-4-methoxydihydrochalcone 2′-O-(3″-O-galloyl)-β-d-glucopyranoside.”
t0“Leea indica (Burm. F.) Merr. Leaves”
Claim · model-written summary
Twenty-four compounds, including three new dihydrochalcones, were first identified from Leea.
LeeaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total 24 compounds including three new dihydrochalcones were identified for the first time from the genus Leea.”