Abstract
Apple stem grooving capillovirus (ASGV) was detected in apple plants in Fraiburgo, Santa Catarina State. The virus was isolated from the cultivars 'Royal Gala' and 'Fuji' grafted onto 'Maruba-kaido", with an MM106 interstock between rootstock and canopy. Two nurseries involving several thousand plants were heavily affected by the virus and had to be destroyed. Declining plants showed tissue necrosis in the 'Maruba-kaido' region around the stem. The virus was readily transmitted to Chenopodium quinoa from flower petals and young leaves of affected plants. In mechanically inoculated Nicotiana occidentalis 37B infection was latent. Virus identification was performed by RT-PCR and ISEM. Key words: stem grooving, apple, identification, PCR, silica capture. Apple stem grooving capillovirus (ASGV) foi detectado em macieiras cv. 'Royal Gala' e 'Fuji' sobre portaenxerto 'Maruba-kaido' com um inter-enxerto de MM 106 entre a copa e o porta-enxerto. Dois viveiros envolvendo milhares de plantas foram afetados severamente e tiveram que ser destruídos. Macieiras em declínio apresentavam necrose da casca no porta-enxerto 'Maruba' ao redor do tronco. O vírus foi facilmente transmitido mecanicamente de pétalas e folhas jovens de macieiras afetadas para C. quinoa e desta para N. occidentalis cv. 37B, na qual o isolado produziu reação latente. A identificação do vírus foi feita por RT-PCR e ISEM.
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Claims 7
Claim · model-written summary
ASGV was detected in apple plants in Fraiburgo, Santa Catarina State.
- Taxon
- apple
Malus domesticae0 - Interactor
- Apple stem grooving capilloviruse0pathogen
- Outcome
- ASGV) was detectede0
- Setting
- Fraiburgo, Santa Catarina Statee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Apple stem grooving capillovirus (ASGV) was detected in apple plants in Fraiburgo, Santa Catarina State”
Claim · model-written summary
The virus was isolated from Royal Gala and Fuji apple cultivars.
- Taxon
- apple
Malus domesticat0 - Material
- 'Royal Gala' and 'Fuji'e0
- Outcome
- virus was isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The virus was isolated from the cultivars 'Royal Gala' and 'Fuji' grafted onto 'Maruba-kaido"”
t0“apple”
Claim · model-written summary
Two nurseries involving several thousand apple plants were heavily affected and destroyed.
- Taxon
- apple
Malus domesticat0 - Outcome
- heavily affected by the viruse0
- Value
- several thousand plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Two nurseries involving several thousand plants were heavily affected by the virus and had to be destroyed”
t0“apple”
Claim · model-written summary
Declining apple plants showed tissue necrosis around the stem in the Maruba-kaido region.
- Taxon
- apple
Malus domesticat0 - Material
- 'Maruba-kaido'e0
- Organ / stage
- steme0
- Outcome
- tissue necrosise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Declining plants showed tissue necrosis in the 'Maruba-kaido' region around the stem”
t0“apple”
Claim · model-written summary
The virus was readily transmitted from affected apple petals and young leaves to Chenopodium quinoa.
- Taxon
- apple
Malus domesticat0 - Organ / stage
- flower petals and young leavese0
- Outcome
- virus was readily transmitted to Chenopodium quinoae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The virus was readily transmitted to Chenopodium quinoa from flower petals and young leaves of affected plants”
t0“apple”
Claim · model-written summary
Mechanically inoculated Nicotiana occidentalis 37B had latent infection.
- Taxon
- Nicotiana occidentalis
Nicotiana occidentalise0 - Material
- Nicotiana occidentalis 37Be0
- Treatment
- mechanically inoculatede0
- Outcome
- infection was latente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“In mechanically inoculated Nicotiana occidentalis 37B infection was latent”
Claim · model-written summary
RT-PCR and ISEM identified the virus in apple.
- Taxon
- apple
Malus domesticat0 - Outcome
- Virus identificatione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Virus identification was performed by RT-PCR and ISEM”
t0“apple”