Paper · 1999 · Americanae (AECID Library)

Occurrence of apple stem grooving capillovirus in Santa Catarina, Brazil, detected by RT-PCR.

Osmar Nickel; W. Jelkmann; G. B. Kuhn

7
claims
12
verbatim quotes
8
citations
3
research areas
✓
Open access · other-oa

Abstract

Apple stem grooving capillovirus (ASGV) was detected in apple plants in Fraiburgo, Santa Catarina State. The virus was isolated from the cultivars 'Royal Gala' and 'Fuji' grafted onto 'Maruba-kaido", with an MM106 interstock between rootstock and canopy. Two nurseries involving several thousand plants were heavily affected by the virus and had to be destroyed. Declining plants showed tissue necrosis in the 'Maruba-kaido' region around the stem. The virus was readily transmitted to Chenopodium quinoa from flower petals and young leaves of affected plants. In mechanically inoculated Nicotiana occidentalis 37B infection was latent. Virus identification was performed by RT-PCR and ISEM. Key words: stem grooving, apple, identification, PCR, silica capture. Apple stem grooving capillovirus (ASGV) foi detectado em macieiras cv. 'Royal Gala' e 'Fuji' sobre portaenxerto 'Maruba-kaido' com um inter-enxerto de MM 106 entre a copa e o porta-enxerto. Dois viveiros envolvendo milhares de plantas foram afetados severamente e tiveram que ser destruídos. Macieiras em declínio apresentavam necrose da casca no porta-enxerto 'Maruba' ao redor do tronco. O vírus foi facilmente transmitido mecanicamente de pétalas e folhas jovens de macieiras afetadas para C. quinoa e desta para N. occidentalis cv. 37B, na qual o isolado produziu reação latente. A identificação do vírus foi feita por RT-PCR e ISEM.

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Claims 7

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

ASGV was detected in apple plants in Fraiburgo, Santa Catarina State.

Interactor
Apple stem grooving capilloviruse0pathogen
Outcome
ASGV) was detectede0
Setting
Fraiburgo, Santa Catarina Statee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Apple stem grooving capillovirus (ASGV) was detected in apple plants in Fraiburgo, Santa Catarina State”

✓ located verbatim in the source · abstract, characters 0–103 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

The virus was isolated from Royal Gala and Fuji apple cultivars.

Material
'Royal Gala' and 'Fuji'e0
Outcome
virus was isolatede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“The virus was isolated from the cultivars 'Royal Gala' and 'Fuji' grafted onto 'Maruba-kaido"”

✓ located verbatim in the source · abstract, characters 105–198 · show in text ↑
t0

“apple”

✓ located verbatim in the source · title, characters 14–19 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Two nurseries involving several thousand apple plants were heavily affected and destroyed.

Outcome
heavily affected by the viruse0
Value
several thousand plantse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Two nurseries involving several thousand plants were heavily affected by the virus and had to be destroyed”

✓ located verbatim in the source · abstract, characters 255–361 · show in text ↑
t0

“apple”

✓ located verbatim in the source · title, characters 14–19 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Declining apple plants showed tissue necrosis around the stem in the Maruba-kaido region.

Material
'Maruba-kaido'e0
Organ / stage
steme0
Outcome
tissue necrosise0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Declining plants showed tissue necrosis in the 'Maruba-kaido' region around the stem”

✓ located verbatim in the source · abstract, characters 363–447 · show in text ↑
t0

“apple”

✓ located verbatim in the source · title, characters 14–19 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

The virus was readily transmitted from affected apple petals and young leaves to Chenopodium quinoa.

Organ / stage
flower petals and young leavese0
Outcome
virus was readily transmitted to Chenopodium quinoae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“The virus was readily transmitted to Chenopodium quinoa from flower petals and young leaves of affected plants”

✓ located verbatim in the source · abstract, characters 449–559 · show in text ↑
t0

“apple”

✓ located verbatim in the source · title, characters 14–19 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Mechanically inoculated Nicotiana occidentalis 37B had latent infection.

Taxon
Nicotiana occidentalisNicotiana occidentalise0
Material
Nicotiana occidentalis 37Be0
Treatment
mechanically inoculatede0
Outcome
infection was latente0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana occidentalis
Nicotiana occidentalis · TaxonJ.W. Wrigley · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In mechanically inoculated Nicotiana occidentalis 37B infection was latent”

✓ located verbatim in the source · abstract, characters 561–635 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

RT-PCR and ISEM identified the virus in apple.

Outcome
Virus identificatione0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Virus identification was performed by RT-PCR and ISEM”

✓ located verbatim in the source · abstract, characters 637–690 · show in text ↑
t0

“apple”

✓ located verbatim in the source · title, characters 14–19 · show in text ↑