Abstract
Bacterial panicle blight caused by Burkholderia glumae is a major bacterial disease of rice. Our preliminary RNA-seq study showed that a serine metalloprotease gene, prtA, is regulated in a similar manner to the genes for the biosynthesis and transport of toxoflavin, which is a known major virulence factor of B. glumae. prtA null mutants of the virulent strain B. glumae 336gr-1 did not show a detectable extracellular protease activity, indicating that prtA is the solely responsible gene for the extracellular protease activity detected from this bacterium. In addition, inoculation of rice panicles with the prtA mutants resulted in a significant reduction of disease severity compared with the wild-type parent strain, suggesting the requirement of prtA for the full virulence of B. glumae. A double mutant deficient in both serine metalloprotease and toxoflavin (ΔtoxA/prtA − ) exhibited a further numeric but not statistically significant decrease of disease development compared with the ΔtoxA strain. Both the prtA-driven extracellular protease activity and the toxoflavin production were dependent on both the tofI/tofR quorum-sensing and the global regulatory gene qsmR, indicating the important roles of the two global regulatory factors for the bacterial pathogenesis by this pathogen.
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Claims 5
Claim · model-written summary
prtA null mutants lacked detectable extracellular protease activity.
- Taxon
- rice
Oryza sativae1 - Interactor
- B. glumae 336gr-1
Burkholderia glumaee0pathogen - Gene
- prtAe0
- Outcome
- extracellular protease activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“prtA null mutants of the virulent strain B. glumae 336gr-1 did not show a detectable extracellular protease activity”
e1“a major bacterial disease of rice”
Claim · model-written summary
Inoculation with prtA mutants significantly reduced rice panicle disease severity versus wild type.
- Taxon
- riceOryza sativae0
- Organ / stage
- paniclese0
- Treatment
- inoculation of rice panicles with the prtA mutantse0
- Gene
- prtAe0
- Outcome
- disease severitye0
- Direction
- reductione0
- Comparator
- wild-type parent straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inoculation of rice panicles with the prtA mutants resulted in a significant reduction of disease severity compared with the wild-type parent strain”
Claim · model-written summary
The double mutant showed a further numeric but nonsignificant disease decrease versus ΔtoxA.
- Taxon
- riceOryza sativae1
- Treatment
- ΔtoxA/prtA −e0
- Outcome
- disease developmente0
- Direction
- decreasee0
- Comparator
- ΔtoxA straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A double mutant deficient in both serine metalloprotease and toxoflavin (ΔtoxA/prtA − ) exhibited a further numeric but not statistically significant decrease of disease development compared with the ΔtoxA strain”
e1“a major bacterial disease of rice”
Claim · model-written summary
Extracellular protease activity depended on tofI/tofR and qsmR.
- Taxon
- riceOryza sativae1
- Gene
- qsmRe0
- Outcome
- prtA-driven extracellular protease activitye0
- Qualifier
- tofI/tofR quorum-sensinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“prtA-driven extracellular protease activity and the toxoflavin production were dependent on both the tofI/tofR quorum-sensing and the global regulatory gene qsmR”
e1“a major bacterial disease of rice”
Claim · model-written summary
Toxoflavin production depended on tofI/tofR and qsmR.
- Taxon
- riceOryza sativae1
- Gene
- qsmRe0
- Outcome
- toxoflavin productione0
- Qualifier
- tofI/tofR quorum-sensinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“toxoflavin production were dependent on both the tofI/tofR quorum-sensing and the global regulatory gene qsmR”
e1“a major bacterial disease of rice”