Abstract
The USDA-ARS National Plant Germplasm System (NPGS) maintains 946 accessions of sugarcane (Saccharum spp.) in the field at the Subtropical Horticulture Research Station in Miami, Florida.These accessions are particularly vulnerable to hurricanes, diseases, and other threats.We sought to identify a method whereby clonally propagated sugarcane accessions could be successfully introduced into tissue culture, multiplied, and then cryopreserved as shoot tips for long-term preservation at the National Laboratory for Genetic Resources Preservation in Fort Collins, Colorado.For many accessions, 70% isopropyl alcohol and 20% commercial bleach treatments, followed by three rinses of sterile water were sufficient to remove microbial contaminants during the introduction process.However, in some cases, cefotaxime was particularly effective for removing bacterial contamination.We found that antioxidant treatments of glutathione, glycine betaine, and ascorbic acid did not improve regrowth after liquid nitrogen exposure using either PVS2 or PVS3 as cryoprotectants in droplet vitrification cryopreservation methods.Exposure durations of PVS2 and PVS3 were optimized, with and without exposure to liquid nitrogen (LN), and shoot tip regrowth levels ranged from 0 to 37% after LN exposure.Published methods for encapsulation-dehydration and V-plate vitrification cryopreservation procedures were tested to determine if acceptable results could be obtained.Using these methods, shoot tip regrowth levels ranged from 0 to 50% after LN exposure.We conclude that the sugarcane cryopreservation methods that we tested are not yet ready for implementation in the NPGS.
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Claims 8
Claim · model-written summary
Alcohol and bleach treatments with water rinses removed microbial contaminants for many accessions.
- Treatment
- 70% isopropyl alcohol and 20% commercial bleach treatments, followed by three rinses of sterile watere0
- Outcome
- microbial contaminantse0
- Direction
- removee0
- Qualifier
- many accessionse0
SaccharumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For many accessions, 70% isopropyl alcohol and 20% commercial bleach treatments, followed by three rinses of sterile water were sufficient to remove microbial contaminants during the introduction process”
t0“sugarcane (Saccharum spp.)”
Claim · model-written summary
Cefotaxime effectively removed bacterial contamination in some cases.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in some cases, cefotaxime was particularly effective for removing bacterial contamination”
t0“sugarcane (Saccharum spp.)”
Claim · model-written summary
Glutathione did not improve regrowth after liquid nitrogen exposure.
- Treatment
- glutathionee0
- Outcome
- regrowthe0
- Direction
- did not improvee0
- Qualifier
- after liquid nitrogen exposure using either PVS2 or PVS3 as cryoprotectantse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antioxidant treatments of glutathione, glycine betaine, and ascorbic acid did not improve regrowth after liquid nitrogen exposure using either PVS2 or PVS3 as cryoprotectants”
t0“sugarcane (Saccharum spp.)”
Claim · model-written summary
Glycine betaine did not improve regrowth after liquid nitrogen exposure.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antioxidant treatments of glutathione, glycine betaine, and ascorbic acid did not improve regrowth after liquid nitrogen exposure using either PVS2 or PVS3 as cryoprotectants”
t0“sugarcane (Saccharum spp.)”
Claim · model-written summary
Ascorbic acid did not improve regrowth after liquid nitrogen exposure.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antioxidant treatments of glutathione, glycine betaine, and ascorbic acid did not improve regrowth after liquid nitrogen exposure using either PVS2 or PVS3 as cryoprotectants”
t0“sugarcane (Saccharum spp.)”
Claim · model-written summary
Optimized PVS2 and PVS3 exposure produced 0 to 37% regrowth after liquid nitrogen exposure.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure durations of PVS2 and PVS3 were optimized, with and without exposure to liquid nitrogen (LN), and shoot tip regrowth levels ranged from 0 to 37% after LN exposure”
t0“sugarcane (Saccharum spp.)”
Claim · model-written summary
Encapsulation-dehydration and V-plate vitrification procedures produced 0 to 50% shoot tip regrowth after liquid nitrogen exposure.
- Treatment
- encapsulation-dehydration and V-plate vitrification cryopreservation procedurese0
- Outcome
- shoot tip regrowth levelse0
- Value
- 0 to 50%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Published methods for encapsulation-dehydration and V-plate vitrification cryopreservation procedures were tested to determine if acceptable results could be obtained.Using these methods, shoot tip regrowth levels ranged from 0 to 50% after LN exposure”
t0“sugarcane (Saccharum spp.)”
Claim · model-written summary
The tested sugarcane cryopreservation methods were judged unready for implementation in the NPGS.
Verbatim
e0“the sugarcane cryopreservation methods that we tested are not yet ready for implementation in the NPGS”
The same questions in other papers
ascorbic acid · growth · SaccharumPositive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.