Abstract
Two efficient and simple cryopreservation methods using aluminium cryo-plates have been developed. In the V cryo-plate method, dehydration is performed using the vitrification solution PVS2, while in the D cryo-plate method, dehydration is achieved using the air current of the laminar flow cabinet or silica gel. To date, more than 20 papers have been published related to both methods. The main advantages of the V and D cryo-plate methods are as follows: handling of specimens throughout the procedure is easy and quick because only the cryo-plates are manipulated. The specimens attached on cryo-plates can be efficiently treated with loading solution (LS) and PVS2/air flow. Cooling and warming are performed easily by immersing the cryo-plates in LN and 1.0 M sucrose solution, respectively, resulting in ultra-rapid cooling and warming rates. High regeneration can be obtained using both methods. For species, which are sensitive to PVS2, the D cryo-plate method can be used. Both methods include preparation of material to be cryopreserved, preconditioning, excision, preculture, mounting of explants on cryo-plates, osmoprotection, dehydration by PVS or air flow, liquid nitrogen storage, rewarming and regeneration. Both protocols appear promising for cryopreservation of both herbaceous and woody plants including tropical plants after appropriate modifications of the procedures. Optimization of the dehydration time, preconditioning of materials and post-cryopreservation regrowth conditions are crucial to achieve high regrowth. These new cryopreservation methods will facilitate the efficient implementation of cryo-storage and long-term maintenance of plant genetic resources in genebanks.
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Claims 8
Claim · model-written summary
V and D cryo-plate methods permit easy, quick handling of specimens.
- Taxon
- herbaceous and woody plantse1
- Outcome
- handling of specimens throughout the procedure is easy and quicke0
Not reportedComparatorValue
Verbatim
e0“The main advantages of the V and D cryo-plate methods are as follows: handling of specimens throughout the procedure is easy and quick because only the cryo-plates are manipulated.”
e1“Both protocols appear promising for cryopreservation of both herbaceous and woody plants”
Claim · model-written summary
Specimens on cryo-plates can be efficiently treated with loading solution and PVS2 or air flow.
- Taxon
- herbaceous and woody plantse1
- Outcome
- efficiently treated with loading solution (LS) and PVS2/air flowe0
Not reportedComparatorValue
Verbatim
e0“The specimens attached on cryo-plates can be efficiently treated with loading solution (LS) and PVS2/air flow.”
e1“both herbaceous and woody plants”
Claim · model-written summary
Cryo-plate immersion allows easy cooling and warming with ultra-rapid rates.
Not reportedComparatorValue
Verbatim
e0“Cooling and warming are performed easily by immersing the cryo-plates in LN and 1.0 M sucrose solution, respectively, resulting in ultra-rapid cooling and warming rates.”
e1“both herbaceous and woody plants”
Claim · model-written summary
Both cryo-plate methods can yield high regeneration.
Not reportedComparatorValue
Verbatim
e0“High regeneration can be obtained using both methods.”
e1“both herbaceous and woody plants”
Claim · model-written summary
The D cryo-plate method can be used for species sensitive to PVS2.
Verbatim
e0“For species, which are sensitive to PVS2, the D cryo-plate method can be used.”
e1“both herbaceous and woody plants”
Claim · model-written summary
Both cryo-plate protocols appear promising for herbaceous and woody plant preservation after modifications.
- Taxon
- herbaceous and woody plantse0
- Outcome
- promising for cryopreservatione0
- Qualifier
- after appropriate modifications of the procedurese0
Not reportedComparatorValue
Verbatim
e0“Both protocols appear promising for cryopreservation of both herbaceous and woody plants including tropical plants after appropriate modifications of the procedures.”
Claim · model-written summary
Dehydration time, preconditioning and regrowth conditions should be optimized for high regrowth.
Verbatim
e0“Optimization of the dehydration time, preconditioning of materials and post-cryopreservation regrowth conditions are crucial to achieve high regrowth.”
e1“both herbaceous and woody plants”
Claim · model-written summary
The authors expect cryo-plate methods to facilitate long-term plant genetic-resource storage.
- Taxon
- plante0
- Outcome
- efficient implementation of cryo-storage and long-term maintenance of plant genetic resourcese0
Not reportedComparatorValue
Verbatim
e0“These new cryopreservation methods will facilitate the efficient implementation of cryo-storage and long-term maintenance of plant genetic resources in genebanks.”