Claim · model-written summary
Cryo-plate immersion allows easy cooling and warming with ultra-rapid rates.
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Verbatim
e0“Cooling and warming are performed easily by immersing the cryo-plates in LN and 1.0 M sucrose solution, respectively, resulting in ultra-rapid cooling and warming rates.”
e1“both herbaceous and woody plants”
Title
Development of V and D cryo-plate methods as effective protocols for cryobanking
T. Niino; S. Yamamoto; T. Matsumoto; et al. Acta Horticulturae 2019. doi:10.17660/actahortic.2019.1234.33
Other claims from this paper (7)
- Method / resource V and D cryo-plate methods permit easy, quick handling of specimens.
- Method / resource Specimens on cryo-plates can be efficiently treated with loading solution and PVS2 or air flow.
- Method / resource Both cryo-plate methods can yield high regeneration.
- Recommendation The D cryo-plate method can be used for species sensitive to PVS2.
- Method / resource Both cryo-plate protocols appear promising for herbaceous and woody plant preservation after modifications.
- Recommendation Dehydration time, preconditioning and regrowth conditions should be optimized for high regrowth.
- Method / resource The authors expect cryo-plate methods to facilitate long-term plant genetic-resource storage.