Abstract
Many abiotic stresses cause the excessive accumulation of reactive oxygen species known as oxidative stress. While analyzing the effects of oxidative stress on tobacco, we noticed the increased accumulation of a specific protein in extracts from plants treated with the oxidative-stress inducing herbicide paraquat which promotes the generation of reactive oxygen species primarily in chloroplasts. The primary objectives of this study were to identify this protein and to determine if its accumulation is indeed a result of oxidative stress. Here we show that the paraquat-induced protein is a covalently linked dimer of the large subunit of ribulose-1,5-bisphosphate carboxylase (LSU). Increased accumulation of this LSU dimer was also observed in tobacco plants exposed to ultra-small anatase titanium dioxide nanoparticles (nTiO 2 ), which because of their surface reactivity cause oxidative stress by promoting the generation of superoxide anion. nTiO 2 nanoparticle treatments also caused a decline in the chloroplast thylakoid proteins cytochrome f and chlorophyll a/b binding protein, thus confirming that covalent LSU dimer formation coincides with loss of chloroplast function.
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Claims 3
Claim · model-written summary
Paraquat increased a protein identified as covalently linked Rubisco large-subunit dimer.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- paraquat-inducede0
- Gene
- large subunit of ribulose-1,5-bisphosphate carboxylasee0
- Outcome
- covalently linked dimere0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the paraquat-induced protein is a covalently linked dimer of the large subunit of ribulose-1,5-bisphosphate carboxylase (LSU)”
t0“tobacco plants”
Claim · model-written summary
Titanium dioxide nanoparticles increased LSU dimer accumulation.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- ultra-small anatase titanium dioxide nanoparticles (nTiO 2 )e0
- Outcome
- LSU dimere0
- Direction
- Increased accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increased accumulation of this LSU dimer was also observed in tobacco plants exposed to ultra-small anatase titanium dioxide nanoparticles (nTiO 2 )”
Claim · model-written summary
Nanoparticle treatments decreased cytochrome f and chlorophyll a/b binding protein.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- nTiO 2 nanoparticle treatmentse0
- Outcome
- cytochrome f and chlorophyll a/b binding proteine0
- Direction
- declinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nTiO 2 nanoparticle treatments also caused a decline in the chloroplast thylakoid proteins cytochrome f and chlorophyll a/b binding protein”
t0“tobacco plants”
The same questions in other papers
nanoparticle · physiology: pigments · Nicotiana tabacum (tobacco)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.