Abstract
BACKGROUND: Tomato torrado virus (ToTV) infection manifests with burn-like symptoms on leaves, leaflets and upper stem parts of susceptible infected plants. The symptoms caused by ToTV may be considered as one of the most severe virus-induced forms of systemic necrosis, which spreads within the whole plant and leads to a lethal phenotype. However, to date there are no data revealing which viral genes encode for a specific pathogenicity determinant that triggers the plant necrotic response for any torradovirus. In this study we evaluated the influence of three coat protein subunits of ToTV: Vp23, Vp26 and Vp35, transiently expressed from a PVX-based vector, and checked their association with the induction of systemic necrosis in infected Solanum lycopersicum L. (cv. Beta Lux), a natural host of ToTV. METHODS: To estimate how ToTV coat protein subunits might contribute in plant response to virus infection we over-expressed the proteins from PVX-based vector in tomato and analyzed enzymatic activities related with plant defense response. By doing protein qualitative analysis performed by mass spectrometry we indicated the PR10 in protein fraction with induced ribonuclease activity. RESULTS: We observed that only the Vp26 enhanced PVX pathogenicity causing severe necrosis of the infected plant. Moreover, we indicated increased RNase and oxidative activities in plants infected with PVX-Vp26 chimeras only. Importantly, we suspected that this increased RNase activity is associated with increased accumulation of PR10 mRNA and products of its translation. CONCLUSIONS: On the basis of the obtained results, we indicated that Vp26 acts as the elicitor of hypersensitive response-like reactions of PVX-Vp26 manifesting with enhanced pathogenicity of the recombined PVX. This might be the first described suspected necrosis determinant of torradoviruses infecting tomatoes.
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Claims 7
Claim · model-written summary
Of the three ToTV coat protein subunits, only Vp26 enhanced PVX pathogenicity, causing severe necrosis of the infected tomato plant.
- Taxon
- Solanum lycopersicum
Solanum lycopersicumt0 - Treatment
- Vp26e0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- Vp26e0
- Outcome
- PVX pathogenicitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“only the Vp26 enhanced PVX pathogenicity causing severe necrosis of the infected plant”
Claim · model-written summary
RNase activity increased only in plants infected with PVX-Vp26 chimeras.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Treatment
- PVX-Vp26 chimerase0
- Interactor
- PVXPotato virus Xe0pathogen
- Outcome
- RNasee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“increased RNase and oxidative activities in plants infected with PVX-Vp26 chimeras only”
Claim · model-written summary
Oxidative activities increased only in plants infected with PVX-Vp26 chimeras.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Treatment
- PVX-Vp26 chimerase0
- Interactor
- PVXPotato virus Xe0pathogen
- Outcome
- oxidative activitiese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“increased RNase and oxidative activities in plants infected with PVX-Vp26 chimeras only”
Claim · model-written summary
The increased RNase activity was suspected to be associated with increased accumulation of PR10 mRNA and its translation products.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Gene
- PR10 mRNAe0
- Outcome
- RNase activitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“this increased RNase activity is associated with increased accumulation of PR10 mRNA and products of its translation”
Claim · model-written summary
Mass spectrometry identified PR10 in the protein fraction with induced ribonuclease activity.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Gene
- PR10e0
- Outcome
- PR10 in protein fraction with induced ribonuclease activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“we indicated the PR10 in protein fraction with induced ribonuclease activity”
Claim · model-written summary
Vp26 acts as the elicitor of hypersensitive response-like reactions of PVX-Vp26 in tomato.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Gene
- Vp26e0
- Outcome
- hypersensitive response-like reactionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“Vp26 acts as the elicitor of hypersensitive response-like reactions of PVX-Vp26”
Claim · model-written summary
Vp26 might be the first described suspected necrosis determinant of torradoviruses infecting tomatoes.
- Taxon
- tomatoesSolanum lycopersicume0
- Outcome
- necrosise0
- Qualifier
- first described suspected necrosis determinant of torradovirusese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This might be the first described suspected necrosis determinant of torradoviruses infecting tomatoes”