Paper · 2019 · Queensland Department of Agriculture and Fisheries archive of scientific and research publications (Queensland Department of Agriculture and Fisheries)

Identification of a new isolate of Banana mild mosaic virus by high throughput sequencing triggers the development of a new diagnostic primer

Marwa Hanafi; Wei Rong; Kathy Crew; et al.

3
claims
4
verbatim quotes
0
citations
2
research areas
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Not open access

Abstract

The Bioversity International Musa Germplasm Transit Centre (ITC) hosts more than 1,500 accessions covering the genetic diversity of the genus Musa. Its objective is to conserve this diversity and supply plant materials to users worldwide. These accessions can be distributed only if virus infection is not detected during indexing. The indexing is carried out by targeted IC-(RT)-PCR for 10 viruses, electron microscopy and symptom observation (De Clerck et al., 2017). One accession (ITC 0763) tested negative by IC-RT-PCR while viral particles were observed by electron microscopy, suggesting the infection by a new virus species or by a divergent viral strain from an existing species.

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Claims 3

Observationobservedoriginal resultmeasured

Claim · model-written summary

One Musa accession (ITC 0763) tested negative by IC-RT-PCR although viral particles were observed by electron microscopy.

Taxon
MusaMusae1
Material
ITC 0763e0
Interactor
viral particlese0pathogen
Outcome
tested negative by IC-RT-PCR while viral particles were observed by electron microscopye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Musa
Musa · Taxon · picture of the genus (one of its species)Forest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“One accession (ITC 0763) tested negative by IC-RT-PCR while viral particles were observed by electron microscopy”

✓ located verbatim in the source · abstract, characters 470–582 · show in text ↑
e1

“covering the genetic diversity of the genus Musa”

✓ located verbatim in the source · abstract, characters 98–146 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

A new isolate of Banana mild mosaic virus was identified by high throughput sequencing.

Interactor
Banana mild mosaic virusBanana mild mosaic virust0pathogen
Outcome
Identification of a new isolatet0

Not reportedTaxon

Verbatim

t0

“Identification of a new isolate of Banana mild mosaic virus by high throughput sequencing triggers the development of a new diagnostic primer”

✓ located verbatim in the source · title, characters 0–141 · show in text ↑
Method / resourceobservedoriginal resultbasis n/a

Claim · model-written summary

A new diagnostic primer was developed for Banana mild mosaic virus following the sequencing.

Interactor
Banana mild mosaic virusBanana mild mosaic virust0pathogen
Outcome
development of a new diagnostic primert0

Not reportedTaxonComparator

Verbatim

t0

“Identification of a new isolate of Banana mild mosaic virus by high throughput sequencing triggers the development of a new diagnostic primer”

✓ located verbatim in the source · title, characters 0–141 · show in text ↑